强心通脉灵对大鼠急性心肌梗死后心室重构的影响

Effects of Qiangxin Tongmai Ling on reconstitution of cardiac ventricle after acute myocardial infarction in rats

目的探讨强心通脉灵对急性心肌梗死(AMI)大鼠心室重构(VR)的干预机制。方法选择Wistar大鼠85只,随机分为强心通脉灵大剂量组(QXLmax组)12只、强心通脉灵小剂量组(QXLmin组)14只、卡托普利组11只、模型组15只和假手术组13只,采取冠状动脉结扎造成AMI模型,术后均予口服给药治疗,模型组及假手术组予以相应的生理盐水。4周后处死,放射免疫法测血浆血管紧张素Ⅱ(AngⅡ)和超氧化物歧化酶(SOD)含量,计算左心室质量指数,光镜下观察心尖组织病理形态学改变,采用ABC染色测TGF-β1的表达量。结果模型组及各给药组AngⅡ水平较高,各给药组较模型组有下降趋势,其中QXLmax组较卡托普利组及QXLmin组更低。模型组及各给药组SOD水平较假手术组降低,QXLmax组及QXLmin组较模型组有升高趋势,左心室质量指数模型组较假手术组显著升高,各给药组较假手术组有升高趋势,QXLmax组较QXLmin组及卡托普利组低。卡托普利组及QXLmin组健存心肌细胞周围胶原组织明显减少,QXLmax组病理改变最轻,偶有少量胶原纤维增生。TGF-β1表达QXLmax组较QXLmin组及卡托普利组低(P<0.01)。结论强心通脉灵可降低AMI后大鼠血浆AngⅡ水平,增加SOD活性,降低左心室质量指数及心肌组织TGF-β1表达,从而平稳AMI后VR的进程。

Objective It is to explore the treatment mechanism of Qiangxin Tongmai Ling （QXL） on cardiac ventricle reconstitution （VR） after acute myocardial infarction （AMI） in rats. Methods 85 cases of Wistar rats were chosen and made into AMI models by tying coronary artery, and were divided into high dosage of QXL group （QXLmax group） 12 cases, low dosage of QXL group （QXLmin group） 14 cases, Captopril group 11 cases, model group 15 cases and sham operated group 13 cases. All the groups were given the drugs by oral use and model group and sham operated group were given normal saline. After 4 weeks all the rats were killed to determine the contents of SOD and Ang Ⅱ and calculate weight index of left ventricle. Cardiac apex tissue was gotten to observe the changes of pathomorphology under light microscope after HE staining, and the expression of TGF - β1 were detected by ABC staining. Results The levels of Ang Ⅱ were high in every group except sham operated group, and the level got to low down in every treatment group, especially in QXLmax group. The levels of SOD were lower in model group and every treatment group than that in sham operated group, but that decreased less in OXLmax group and OXLmin group. Calculate weight index of left ventricle in every group was higher than that in sham operated group, especially in model group. But the index in OXLmax ,group was lower than that in OXLmin group and Captopril group. The collagen tissue around survival healthy heart muscle cells obviously decreased in OXLmin group and Captopril group, but the pathology changes in OXLmax group were the slightest with less collagen tissue hyperplasia. The expression of TGF- β1 in OXLmax group was less than that in QXLmin group and Captopril group （P〈0.01）. Condnsion QXL can lower down the level of plasma Ang Ⅱ and weight index of left ventricle, decrease the expression of TGF- β1 in heart muscle tissue, and increase the activity of SOD, so to steady the process of VR after AMI in rats.