用植物酯酶法快速检测蔬菜中的有机磷农药

Rapid Determination of Organophosphorus Pesticide in Vegetables With Plant-esterases

为了快速检测蔬菜中的有机磷农药残留状况,分别从京9428、京9583、京东8、京2小麦中提取植物酯酶,将这些酶用于有机磷农药的检测,采用分光光度法比较了不同质量浓度的对硫磷对小麦酯酶活力的抑制情况.结果表明,不同品种小麦酯酶对于对硫磷的敏感度不同,在所研究的小麦酯酶中,京2酯酶对对硫磷最为敏感,其检测限达到3.0×10-7mg/L.同时对酶促反应的温度、时间和pH值进行了优化,并应用此方法对蔬菜样品中的有机磷农药进行了检测.

In order to rapidly determine the organophosphorus pesticide residue in vegetables, esterases are extracted from four types of wheat, i.e. Jing No. 9428, jing No. 9583, JingDong No. 8 and Jing No. 2. The inhibition rates of the esterase activity under different concentrations of parathion are compared by the colorimetric measurement. The results indicate that the inhibiting sensitivities to parathion of esterase extracted from different wheat are not the same. The esterase from the wheat of Jing No. 2 is more sensitive to parathion than that from others, with detection limit of 3.0 ×10^-7 mg/L. Simultaneously, the temperature, time and pH of the enzymic reactions are optimized. Organophosphorus pesticide residues in vegetables are determined by the proposed method.