菜籽壳中单宁提取技术研究

Study on Extraction of Tannin in Rapeseed Shell

本文确立了简单有效的菜籽壳中单宁提取、测定方法,通过对菜籽壳中单宁的提取两有影响的相关因素单因素实验,确定关键影响因素,在此基础上通过响应曲面法优化菜籽壳中单宁提取的最佳工艺条件。利用DesignExpert软件,对油菜籽壳单宁提取率二次多项式数学模型解逆矩阵得知:在pH、提取温度、提取时间和料液比分别为3.00、65.48℃、67.11和20g/ml时,菜籽壳单宁提取量可达到最大值为9.15mg/g。

A kind of method was established on extraction and mensuration method of tannin in rapeseed shell in this paper. Our previous work has indicated that extraction for tannin in rapeseed shell was strongly affectnd by many internal and external factors. By single-factor experiment, we found four critical internal factors： temperature, pH, ratio of material to water and time. In the second phase of the optimization process, a response surface methodology（RSM） was used to optimize the above critical internal factors, and to find out the optimat concenu＇ation levels and the relationships between these factors. Based on the results of the single-factor, a three-level four-factor（temperature, pH, ratio of materiel and water and time） CCD design was employed, By solving the quadratic regression model equation using appropriate statistic methods, the optimal concentrations for obtain 9. 15mg/g extraction for tannin was calculated as： pH3.00, 20g/ml ratio of materiel and water, 65.48℃ and 67.11 minutes. The experimental data under various conditions have validated the theoretical values.