桑黄多糖分离纯化及其结构初步鉴定

Segregation and Purification of Polysaccharides from Phellinus igniariu and Structure Appraisal of This Fraction

本文用离子交换和凝胶层析对桑黄多糖进行了纯化,并对多糖的结构、组成及理化常数进行了分析。结果表明,经弱碱性阴离子和弱酸性阳离子交换树脂一次串联脱蛋白,蛋白脱除率达94.96%,多糖回收率为77.77%。再经凝胶层析,得到大分子量多糖组分HHM(2.84×106Da)和小分子量多糖组分HLM(5.33×104Da),HLM和HHM的旋光度分别为[α]D25=+64.8°和[α]D25=+58.4°,由IR分析,初步推测该HHM和HLM多糖为β型吡喃多糖,由TLC法测得的HHM的单糖组成为葡萄糖,HLM单糖组成分别为葡萄糖和半乳糖。

In this dissertation, we studied anion and cation exchange and to purification of polysaccharides from Phellinus igniariu. The polysaccharide structural and some physicochemical characters of this fraction were researched. Based on experimental results and general consideration, it was suititable to adopt using anion and cation exchange resin in series once. After that, 94.96% protein of crude polysaccharides could be removed, the recovery rate of polysaccharide was 77.77%. Higher molecular weight （HHM） and lower molecular weight（HLM） were gained by gel chromatograph. The optical rotation degree ofPhellinus lgniariu polysaccharides [α]D^25=＋64.8°, [α]D^25=＋58.4°. Finally we study the structural of polysaccharides through IR, the results showed that the main polysaccharide chain was β-pyran-polysaccharide. Besides the monose of HLM through TLC analysis are glucose and galactose. The monose of HHM through TLC analysis are glucose.