香菇富锌及其培养条件优化

Study on Optimization of Cultivation Condition in Zinc-enriched Lentinula edodes

对香菇双单杂交菌株QS29、NN22、QQ06、QQ05进行了富锌培养,确定了其生长耐受锌的浓度范围为0.05～0.3mg/ml。选择香菇菌株NN22进行了培养条件优化与液体出菇试验,结果表明:香菇菌株NN22在PDY培养基中富锌的适宜培养条件为0.1mg/mlZn2+,25℃,pH7.0;在此条件下,1g菌丝(干重)的锌含量达到9.26mg,多糖含量为5.19mg;富锌香菇菌株NN22在0.1mg/mlZn2+PDY培养基里第19d开始出现菇蕾,较未富锌菌株出菇时间缩短了6d。

Four strains（QS29, NN22, QQ06, QQ05） of di-mon mating of Lentinula edodes were Zinc-enriched in the medium with Zn^2＋ content ranged from 0,05 to 0.3mg/ml. The optimization of cultivation conditions of the strain NN22 was selected, and primodium test of the Zinc-enriched swain NN22 was carded out in Zn^2＋ of PDY. The results showed that the highest Zn^2＋ content of the strain NN22 was obtained within 20 clays at 25℃ and initial pH7.0 in 0. lmg/ml Zn^2＋ of PDY. Under the optimized cultivation conditions, Zn^2＋ content and polysaccharide content of the swain NN22 reached 9.26mg/g and 5.19mg/g, respectively. The phase of primodium formed of the Zinc-enriched strain NN22 was 19 days in 0.1mg/ml Zn^2＋ of PDY, which was reduced 6 days compared to the original strain.