上皮性卵巢肿瘤中p53、nm23和增殖细胞核抗原的免疫组化检测及意义

EXPRESSION OF p16 PROTEIN IN HUMAN LUNG CARCINOMAS AND ITS SIGNIFICANCE

采用免疫组化ＬＳＡＢ法检测３５例卵巢上皮性肿瘤组织中ｐ５３、ｎｍ２３和增殖细胞核抗原（ＰＣＮＡ）的表达。结果显示：ｐ５３、ｎｍ２３和ＰＣＮＡ表达的阳性率均以卵巢囊腺癌为最高，交界性次之，良性囊腺瘤最低。各项检测结果与细胞增殖状态有关。ｐ５３和ＰＣＮＡ过量表达与肿瘤恶性程度、临床分期和生存期无关，但与肿瘤分化有关。ｎｍ２３不能单独作为判断卵巢肿瘤预后的因素。

The expression of p16 protein in human lung carcinoma and normal tissues adjacent to it was studied immunohistochemically.Cytoplasmic p16 expression was mainly present in normal bronchial epithelium(93 8%),serous gland (92 6%) and alveolar epithelium(71 4%).The expression levels of p16 were significantly lower in carcinoma tissues (66 1%) compared to those in their normal counterparts( P <0 01).Squamous cell carcinoma exhibited lower p16 expression levels compared with adenocarcinoma( P <0 01).The expression levels of p16 were lower in poorly differentiated carcinoma than those in well differentiated carcinomas( P <0 05).In adenocarcinomas,no relationship was found between p16 expression and stages,but decreases in p16 expression were significant in advanced stages of squamous cell carcinomas.These results suggest that deletion of p16 protein expression is related with carcinogenesis of lung carcinomas,and becomes more evident as tumor slows down its differentiation and advances in conditions.