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尿酸对HBV表位肽S_(28-39)负载DC诱导免疫效应的影响

Effects of uric acid on the immune responses induced by dendritic cells pulsed with S_(28-39) peptide of HBV epitope
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摘要 目的观察尿酸对由HBV表面抗原表位肽负载的树突状细胞诱导免疫效应的影响。方法以负载肽S28-39的小鼠骨髓来源树突细胞,联合尿酸(uric acid,UA)尾静脉注射接种小鼠,每周1次,共2次。分为DC对照组,联合尿酸组,尿酸对照组,正常对照组。流式细胞仪法检测特异性CTL活性;ELISA法检测小鼠脾细胞培养上清液IFN-γ水平。结果体内S28-39特异性CTL,以联合尿酸接种组杀伤活性最强;联合尿酸接种组脾细胞IFN-γ分泌较对照组增高。结论尿酸能够增强乙肝表位肽负载的树突细胞诱导的S28-39特异性CTL杀伤活性;能促进免疫小鼠脾淋巴细胞分泌IFN-γ。尿酸具有增强负载S28-39树突细胞诱导的细胞免疫效应的活性。 Objective To evaluate the immune response in Balb/c mice immunized with uric acid and dendritic cells （DCs） pulsed with a HBsAg epitope peptide S28-29. Methods Balb/c mice were immunized with DC pulsed with the S28-29 peptide （S28-29-pulsed-DCs）, combined with uric acid. DCs and uric acid were injected through the tail vein of each mouse, and the immunization was repealed after 7 days. Cytotoxicity of specific CrLs in vivo was determined by the CFSE labeled spleen assay. Production of IFN-γ in the supematants of cultured splenoeytes was determined by using a commercial ELISA kit. Results The specific CTLs induced by S28-29 B-pulsed-DCs and uric acid had strong cytotoxicity to S28-29 Production of IFN-7 was increased in mice immunized with S28-29B-pulsed-DCs and uric acid. Conclusion Uric acid can promote the S28-29 specific cytotoxicity and the production of IFN-γ induced by S28-29 peptide pulsed-DCs;uric acid can enhance the cellular immune response induced by S28-29-pulsed-DCs.
作者 马晓军 田德英 贺杰峰 许东 梁智辉 李晖
机构地区 华中科技大学同济医学院附属同济医院感染科 华中科技大学同济医学院附属同济医院普外科 华中科技大学同济医学院免疫学系
出处 《免疫学杂志》 CAS CSCD 北大核心 2007年第1期26-29,共4页 Immunological Journal
基金 国家自然科学基金(30471533) 湖北省自然科学基金项目(2006ABA139)资助
关键词 尿酸 树突状细胞 乙型肝炎表面抗原表位肽 细胞毒性T淋巴细胞 Uric acid Dendritic cells Peptide of HBV surface antigen epitope Cytotoxic T lymphocytes
分类号 R392 [医药卫生—免疫学]
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参考文献10

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二级参考文献8

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免疫学杂志
2007年 第1期

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