摘要
观察中药肺宁对博莱霉素诱导的小鼠肺纤维化的治疗作用,以博菜霉素气管内注入建立肺纤维化模型,次日始分别给予中药肺宁和强的松灌胃治疗,于给药后三个月处死动物,右肺组织行病理组织学检查,左肺组织匀浆进行丙二醛(MDA)、谷胱甘肽(GSH)、肿瘤坏死因子(TNF-α)含量检测。结果与模型组相比,肺宁各剂量组、强的松组的MOA、TNF-α含量显著降低(P<0.05);肺宁高、中剂量组的GSH含量显著升高(P<0.05)。各治疗组相比,肺宁高剂量组TNF-α含量低于肺宁低剂量组(P<0.05);肺宁高剂量组GSH含量高于肺宁中、低剂量组(P<0.05);肺宁中剂量组GSH含量高于肺宁低剂量组(P<0.05)。证明肺宁可使肺损伤大鼠肺组织匀浆中TNF-α水平下调,减轻脂质过氧化反应,清除自由基抑制自由基生成,并削弱炎症反应干扰肺纤维化进程。
To study the effect of Chinese medicine Feining on experimental pulmonary fibrosis in rats induced by Bleomycin （ BL）, all rats were randomly divided into control groups, model groups, Feining groups （ dose ： 1 g/kg;2 g/kg ;4 g/kg）, prednisone groups and given BL endotracheally except the normal control group. On day 2, each BL group was given Feining and prednisone respectively except BL control group. After three months, 10 rats in each group were killed and lungs were harvested. The right lungs were subjected to histopathological studies, the left lungs were homogenized for biochemical assays of MDA, GSH, TNF-α content. Compared to model group, MDA, TNF-α contents of homogenized lungs in each Feining group and prednisone group decreased （ P 〈 0. 05 ）, GSH content of Feining （4 g/kg ;2 g/kg）group and prednisone group increased （P 〈 0. 05 ）. TNF-α contents of Feining（4 g/kg） group decreased as compared with Feining（ 1 g/kg）group and GSH increased as compared with Feining（2 g/kg; 1 g/kg）group, GSH contents of Feining（2 g/kg）group increased as compared with Feining（ 1 g/kg） group. It is conclused that Feining can reduce the contents of TNF-α ;reduce lipid peroxide（LPO） mediated injury; enhance the power of eliminating oxygen radical. So, Feining can alleviate pulmonary alveolitis; resist pulmonary fibrosis.
出处
《科学技术与工程》
2007年第3期277-280,共4页
Science Technology and Engineering
基金
军队医药卫生科研基金项目(06MA123)资助
