HPLC测定御感袋泡茶中葛根素和黄芩苷的含量

Determination the contents of baicalin and puerarin in Yugan Daipaocha by HPLC

目的：采用高效液相色谱法测定黄芩、葛根及其制剂御感袋泡茶中黄芩苷和葛根素的含量，评价该制剂的质量。方法与结果：黄芩苷测定以ＫＹＷＧ－Ｃ１８为固定相，０．５％磷酸－甲醇－Ｎ，Ｎ－二甲基甲酰胺（１３∶１０∶１）为流动相，ＵＶ检测波长为２７６ｎｍ，黄芩苷在０．１～０．０１ｍｇ／ｍｌ浓度范围与峰面积呈直线关系，回归方程为Ｙ＝０．００１８＋９．９５×１０－８Ｘ，ｒ＝０．９９９６，平均回收率为１０２．８％，ＲＳＤ为１．８６％（ｎ＝５）。葛根素测定以ＫＹＷＧ－Ｃ１８为固定相，水－甲醇－Ｎ，Ｎ－二甲基甲酰胺（４０∶１０∶３）为流动相，ＵＶ检测波长为２５０ｎｍ，葛根素在０．２～０．０２０ｍｇ／ｍｌ浓度范围与峰面积呈直线关系，回归方程为Ｙ＝１．５×１０－４＋１．０９×１０－７Ｘ，ｒ＝０．９９９９，平均回收率为１０１．７％，ＲＳＤ为１．６２％（ｎ＝５）。结论：本法简便、快速。

OBJECTIVE:To evaluate the quality of baicalin in Scutellaria baicalensis Georgi,puerarin in Puerarisa lobata(Willd.) is Yagan Baipaocha by high performence liquid chromatgraphy.METHOD:Baicalin was separated on KYWG C 18 column and detected at 276 nm,by using 5 g/L phosphoric acid methanol N,N dimethylformamide(13∶10∶1) as mobile phase.RESULTS:Puerarin was separated on KYWG C 18 column and detected at 250 nm by using water methanol N,N dimethylformamide(40∶10∶3) as mobile phase.There was a good linearity from 0.1 to 0.01 mg/ml(r=0.999 6,Y=1.8×10 -3 +9.95×10 -8 X) in separation of baicalin.The average recovery was 102.8% and the relative standard deviation 1.86%(n=5).There was a good linearity from 0.2 to 0.020 mg/ml(r=0.999 9,Y=1.5×10 -4 +1.09×10 -7 X) in separation of puerarin.The average recovery was 101.7 % and the relative standard deviation 1.62%(n=5).CONCLUSION:This method is simple and fast,and the results are reliable.