摘要
目的:探讨重组内皮抑素(Endostatin)对碱烧伤诱导的兔角膜新生血管(corneal neovasculariza-tion,CNV)的抑制作用。方法:选取新西兰大白兔18只,采用碱烧伤法制备兔CNV模型,随机分为3组,每组6只12眼。2个实验组分别以10μg/ml和50μg/ml重组内皮抑素点眼,对照组用生理盐水点眼。定期摄相,采用计算机图像分析系统比较CNV生长面积。结果:与对照组相比,2个实验组自第7 d开始CNV平均生长面积明显减少(P<0.05),经角膜组织病理学检查,新生血管明显稀少,但炎性细胞成分及浸润程度与对照组相似。结论:10、50μg/ml重组内皮抑素具有明显抑制角膜新生血管生长的作用,无毒副作用,为临床防治CNV提供了一种新的途径。
Objective: To investigate the effects of recombinant human endostatin on corneal neovascularization (CNV). Methods: Alkanli bum was used to induce neovascularization in 18 New Zealand white rabbits, which were divided into 3 groups at random, 6 in each. 10 μg/ml and 50μg/ml recombinant human endostatin were used respectively in group 1 and 2, and normal saline in group 3 (control). The area Of CNV was determined by computer imaging analysis system. Results: The mean area of CNV of group 1 and 2 was significantly smaller than that of the control group from the 7th day( P 〈0.05). No significant difference was found between group 1 and 2. Conclusion: Recombinant human endostatin has prominent effect in inhibiting the corneal angiogenesis.
出处
《山东大学耳鼻喉眼学报》
CAS
2006年第6期552-554,558,共4页
Journal of Otolaryngology and Ophthalmology of Shandong University
基金
山东省医药卫生科研项目(2001CA2CAA1)
关键词
内皮押素
烧伤
化学
角膜新生血管
Endostatin
Bum, chemical
Comeal neovascularization