旋毛虫活体传代技术的研究

Exploration on Living Passage for Trichinella Isolates

目的建立标准化旋毛虫的活体传代技术为教学和科研服务。方法取保种用旋毛虫各隔离种肌幼虫,经口感染健康小鼠,于感染40 d后剖杀小鼠。取躯体肌肉用组织捣碎机将肌肉绞碎,按要求的比例加入人工胃液,于37℃温箱中消化46～h。然后将消化液反复洗涤沉淀,用贝尔曼氏装置收集旋毛虫肌幼虫,在光学显微镜下观察旋毛虫各隔离种的形态。结果在显微镜下可见卷曲如螺旋状的活虫体。4个旋毛虫隔离种对小鼠的感染性显著差异(P<0.01),猪旋毛虫和旋毛形线虫(T.spiralis)的感染性较强,在小鼠体内的繁殖力指数(RCI)分别为121.01±7.80和149.86±7.47;而犬旋毛虫和本地毛形线虫(T.nativa)易感性差,RCI分别为60.98±5.05和55.15±4.69。结论用消化法进行的旋毛虫活体传代效果好,可永久保存。

Objective To establish a standard technique on passaging TrichineUa isolates for the purpose of teaching and scientific research. Methods Healthy mice were orally infected with muscle larvae of Trichinella isolates, and mice were killed at 40 days post infection. The weighed muscles of mice were minced into small pieces and digested by artificial gastric juice. The minced muscle tissues were incubated at 37℃ for g to 6 hours. The larvae were collected from the supematant after appropriate filtration and differential sinking. The morphology of Trichinella isolates was observed by the microscope. Result The worms curled tightly like spiral in the microscope. Infectivity of Trichinella from swine and dog, T.spiralis and T.nativa in mice was significantly different （P〈0.01）. Swine isolate and T.spiralis were highly infective to mice with RCIs of 121.01 and 149.86 respectively. However, mice was relatively refractory to dog isolate and T.nativa with RCIs of 60.98 and 55.15 respectively. Conclusion The effect is fine to passage by the way of digestion, and it can be preserved enduringly.