我国牛羊梨形虫病病原的收集与鉴定

Collection and Identification of Piroplasma Infected to Cattle and Sheep in China

目的建立标准化的梨形虫病病原收集与鉴定技术,用于我国牛羊梨形虫病的防治研究。方法自野外采集动物血液或媒介蜱,根据形态进行蜱种鉴定,实验性感染除脾动物,对我国不同地区、不同家畜的梨形虫病病原进行分离,并通过蜱传播试验确定媒介蜱的种类与传播方式;采用18S rRNA基因测序方法,对本实验室已分离的部分梨形虫进行进化关系研究。结果获得34个牛和羊的梨形虫不同地区分离株,并对病原进行了分类鉴定。结论采用血液注射或蜱传播试验可对梨形虫进行有效分离,通过传统分类学和病原进化关系研究,可对动物梨形虫进行正确的分类鉴定。

Objective To establish a standard method on collection and identification of Piroplasma infected to cattle and sheep. Methods The Piroplasma were isolated by inoculating the blood or tick collected from the field, the tick vectors were determined by transmission experiment; The phylogenetic relationship was studied by sequencing 18S rRNA gene. Results Thirty four isolates of Piroplasma were obtained from the distinct areas of China, and some studies on their taxonomy were carried out. Conclusion Piroplasma could be effectively isolated by the blood inoculation or by the transmission with ticks, more accurate taxonomic studies were obtained by the classical method in combination with the molecular method.