摘要
本研究利用基因重组技术,构建并表达吉布森巴贝斯虫的重组顶膜抗原-1(BgAMA-1),将BgAMA-1作为诊断抗原,建立针对吉布森巴贝斯虫的ELISA诊断方法。结果显示,构建的重组表达质粒得以表达,建立的ELISA方法在血清学诊断中有较好的特异性。
In order to improve diagnostic method to Babesia gibsoni infection, gene recombination technique was used in our study. By this technique, we constructed and expressed recombinant apical membrane antigen-1 （BgAMA-1）, and recombinant BgAMA-1 was used in ELISA experiment as diagnostic antigen. The results showed the recombinant expressed plasmid was triumphantly constructed and the recombinant antigen BgAMA-1 had a high specificity in ELISA test. These results demonstrate that the recombinant BgAMA-1 might be a useful diagnostic reagent for detection of antibodies to B.gibsoni in dogs.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2006年第B12期62-64,共3页
Chinese Journal of Parasitology and Parasitic Diseases
