心理应激小鼠脑区核团原癌基因蛋白表达的规律及加味逍遥丸调节作用的实验研究

Rules of Protein Expression of Proto-oncogene (c-Fos/c-Jun) in Different Brain Areas and Nucleus of Psychological Stressed Mice and the Regulatory Effect of Modified Xiaoyao Pill

目的探讨心理应激后小鼠脑区原癌基因(c-Fos、c-Jun)蛋白表达的规律及加味逍遥丸对该蛋白表达的调节作用。方法利用电刺激心理应激箱诱发小鼠心理应激动物模型,加味逍遥丸(2mg/g体重)灌胃给药,免疫组化法检测c-Fos、c-Jun蛋白,观察不同脑区核团:海马1-4区(hippocampus CA1-4)、杏仁中央核(central amygdaloid nucleus,CAN)、下丘脑室旁核(paraventricular nucleus,PVN)、下丘脑视上核(supraoptic nucleus,SON)的表达变化。结果与正常组比较,应激1次后3h组、应激3次后3h组、应激5次后3h组各脑区c-Fos、c-Jun蛋白表达均增强(P<0·01);与应激1次后3h组比较,应激1次后1h组和应激1次后6h组表达减弱(P<0·05或P<0·01);与不服药组比较,服药组应激3次后3h组和应激5次后3h组c-Fos、c-Jun蛋白表达均减弱(P<0·05或P<0·01)。结论心理应激后1hc-Fos、c-Jun蛋白开始升高,3h达高峰,c-Fos蛋白6h已回落近正常水平,c-Jun蛋白6h仍维持在较高水平,多次应激后在同一高峰点未见表达减弱现象;加味逍遥丸对应激后c-Fos、c-Jun蛋白的高表达有明显下调作用。

Objective To study the rules of protein expression of proto-oncogene （c-Fos/c-Jun） in different brain areas and nucleus of psychological stressed mice and the regulatory effect of modified Xiaoyao Pill （SXP） Methods The mouse psychological stress model was established by electrical stimulation; SXP was administrated at the dose of 2 mg/g; the protein expression of c-Fos and c-Jun in different brain areas and nucleus, including hippocampus （CA1 - 4）, central amygdaloid nucleus （CAN）, paraventricular nucleus （PVN） and supraoptic nucleus （SON）, were detected by immunohistochemical method. Results The protein expressions of c-Fos and c-Jun in all the tested brain areas of model mice 3 h after being stressed for 1, 3 or 5 times, were significantly higher than those of the normal mice （P〈0.01） ; After being stressed for one time, the expression at 1 h after stimulation was lower than that at 3 h after stimulation （ P 〈 0.05）, the expression in mice treated with SXP was lower than that in the untreated group, 3 h after three or five times of stress （ P 〈 0.05 or P 〈 0.01）. Conclusion The protein expression of c-Fos and c-Jun began to rise 1 h after psychological stress and reached the peak at the 3rd h. That of c-Fos dropped to the normal level approximately after 6 h, but the dropping did not happen in expression of c-Jun, it remained on the high level unweakened after repeated stimulation; SXP can remarkably down-regulate the proto-oncogene （c-Fos and c-Jun） expressions after psychological stress in mice.