固精丹对急性肾功能衰竭模型的保护作用

Protection of Gujingdan on Acute Renal Failure Model

目的:研究中药复方固精丹对庆大霉素致大鼠急性肾功能衰竭的保护作用。方法:将大鼠随机分成空白组、模型组、固精丹高、中、低(4、2、1g/kg)剂量组和阳性对照组(肾康宁2g/kg)。除空白组外,其余各组均腹腔注射庆大霉素(100mg/kg-1.d-1),连续7天;于注射庆大霉素的第2天,除空白组和模型组其余各组分别灌胃固精丹和肾康宁,连续灌胃7天,在末次给药后4h,接24h尿测尿蛋白和N-乙酰-β氨基葡萄糖苷酶(NAG);眶静脉采血测血肌酐(SCR)、尿素氮(BUN);取肾脏作光镜和电镜检查。结果:固精丹治疗组大鼠的尿糖、NAG及肌酐、尿素氮、明显低于模型组;肾脏病理:光镜和电镜检查结果,固精丹高剂量和中剂量组明显好于模型组和阳性对照组。结论:固精丹对庆大霉素引起的急性肾功能衰竭具有保护作用。

Objective： To study the protective effect of Gujingdan on the acute renal failure caused by gentamlcin in rats. Methods： 90 rats were randomly divided in to six groups which are normal control group , pathologic model group , groups treated by Gujinsdan （4,2,1g/kg） and positive control group treated by Shenkangning （2g/kg）. Except normal group the other groups were injected Gentamicin by abdominal cavity（ 100mg· kg^-1· d^-1 ）and at the same time, Gujinsdan and shenkangning were given by ig for seven days. When experiment over to examine the content of urinary protein, β-N-acetyglocosamidase （NAG） ; serum creatinine（SCR） and blood urea nitrogen（ BUN）; the renal histological changes were observed by light and electron microscope. Results： the content of NAG, BUN, SCR of the groups treated by Gujingdan were significantly lower than that of the pathologic model group;The histological changes show that the group treated by Gujingdan were obviously alleviated than that of model and positive control group. Conclusion ：Gujingdan has the function to protect acute renal failure induced by gentamicin.