调脂积冲剂对肝纤维化大鼠肝纤维化指标的影响

The research of Tiaozhiji emulsion on indexes of hepatic fibrosis in rat hepatic fibrosis

目的研究调脂积冲剂的抗肝纤维化作用并评价其疗效。方法清洁级Wister雄性大鼠48只,随机分为4组各12只,每日上午模型对照组、调脂积组、易善复组每只按1 ml/100 g体重灌服乳剂,正常对照组灌服等量蒸馏水,并每间隔2日按每只0.2 ml/100 g体重给模型对照组、调脂积组、易善复组腹腔注射25%四氯化碳(CCl4)油液1次,正常对照组注射等量0.9%氯化钠注射液。每日下午按1.5 ml/100 g体重给调脂积组、易善复组灌服调脂积药液,模型对照组和正常对照组灌服等量蒸馏水,连续8周。第8周末末次给药后禁食,应用放射免疫法测定大鼠血清透明质酸(HA)、Ⅲ型前胶原(PCⅢ),光镜下行肝组织切片病理学观察。结果调脂积组可显著降低血清HA、PCⅢ[(141.52±3.81)ng/ml、(44.82±1.26)ng/ml],与易善复组[(143.04±12.37)ng/ml(、45.07±1.21)ng/ml]比较无显著性差异(P>0.05);光镜下显示调脂积组炎症活动度、肝纤维化程度较模型组轻(P<0.05),与易善复组比较无显著性差异(P>0.05)。结论调脂积冲剂具有良好的抗肝纤维化作用。

Objective To search Tiaozhiji emulsion on indexes of hepatic fibrosis in rat hepatic fibrosis. Methods 48 male Wistar mrs were randomly divided into 4 groups. They were control group, model group, Eseentiale N group and Tiaozhiji emulsion group. Emulsion were taken orally in rats in model group, Eseentiale N group and Tiaozhiji emulsion morning every day, and 0.2 ml/100g 2.5% CCl4 was injected a time three days. Distilled water was taken orally in control group. Afternoon 1 .S ml/100 g Eseenfiale N, Tiaozhiji emulsion were orally taken in Essentiale N group and Tiaozhiji emul- sion group. Distilled water was taken orally were orally taken in control group and model group. The therapeutic course was 8 weeks in four groups. Administration was terminated in 8 weeks. HA and Pcm in rat serum were assayed by radioimmu- noassay. Pathology was observed by light microscope. Results HA and Pcm in rat serum in Tiaozhiji emulsion group were obviously lower than those in model group （ P 〈 O. 05）. There were nor significant differences between Tiaozhiji emulsion group and Eseentiale N group （ P 〉 0.05）. Coclusion Tiaozhiji emulsion has a good effect on anti-fibrosis of hepatic.