妥泰对宫内缺血Wistar大鼠海马星形胶质细胞及学习记忆能力的影响

Effect of Topamax on hippocampal astrocytes and the memory and learning capacity after perinatal ischemic brain damage in Wistar rats

目的观察妥泰对宫内急性脑缺血损伤的Wistar大鼠海马星形胶质细胞及学习记忆能力的影响。方法夹闭足月妊娠大鼠子宫血管,制成急性脑缺血损伤的新生鼠模型,治疗组给予妥泰,观察脑缺血后再灌注3h、6h、24h、3d、7d、14d、21d、28d海马GFAP标记的星形胶质细胞的变化,生后28d通过Morris全自动水迷宫实验,比较各组新生鼠学习记忆能力的差别。结果妥泰治疗组新生鼠反应性星形胶质细胞在脑缺血再灌注早期的增生程度以及继之发生的大量减少的程度均较缺血对照组明显降低(P<0.05),多次给药组可以减少其晚期的过度增生(P<0.05),妥泰治疗组新生鼠学习记忆能力明显好于缺血对照组(P<0.05),且多次给药组学习记忆能力好于单次给药组(P<0.05)。结论妥泰可以明显减少宫内急性脑缺血后新生大鼠星形胶质细胞的异常变化,改善急性脑缺血损伤的Wistar大鼠生后的学习记忆能力。

Objectives To investigate the effect of Topamax on hippocampal astrocytes and the memory and learning capacity after perinatal ischemic brain damage in Wistar rats. Methods The model of perinatal acute ischemic brain damage was established by bilateral uterine artery ligation of full-term pregnant Wistar rats. The effects of Topamax were observed according to the pathological change of GFAP labeled-astrocytes at different time points, including postnatal 3 h, 6 h, 24 h, 3 d, 7 d, 14 d, 21 d and 28 d. The capacity of memory and learning was evaluated by using Morris water maze on the 28th day after birth. Results Neonatal rats treated with Topamax had a lower degree of astrocyte hyper plasia in the early stage of ischemic-reperfusion damage and a minor reduction of astrocytes subsequently （P 〈 0.05） . Excessive hyperplasia in the late stage was decreased after multiple-administration of Topamax （P 〈 0.05） . The memory and learning capacity in the Topamax treated-group was hetter than the control group （P 〈 0.05） . The memory and learning capacity in the multiple-administration group was better than the single-administration group （P 〈 0.05） . Conclusions Topamax could remarkably lessen the pathological change of astrocytes and improve the memory and learning capacity of neonatal rats after perinatal acute ischemic brain damage.