摘要
【目的】探讨耐氟康唑白念珠菌基因多态性与其耐药性是否相关。【方法】收集48株敏感白念珠菌和10株耐氟康唑白念珠菌,其中包括2株体外诱导的耐氟康唑白念珠菌。选择1个随机引物(引物1251),采用任意引物PCR方法基因分型,将电泳图谱扫描入计算机后采用Labwork4.0软件转化为数值图表,利用SPSS11.5进行聚类分析。【结果】引物1251的PCR指纹图带型稳定,多态性丰富,可以作为分型引物。聚类分析结果提示8株临床耐药菌的PCR指纹图相似系数为71.7%,而其中7株的耐药菌相似系数高达86.7%,远高于58株菌的平均相似系数(47.3%)。2株体外诱导的耐药菌诱导前后基因型未发生变化。【结论】基因分型的结果未提示耐氟康唑白念珠菌存在一定的特殊电泳条带,但提示了特定的PCR指纹图可能与耐药性有一定相关性。
[Objectives] To investigate the possible relationship of C. albicans gene polymorphisms and its flueonazole-resistance. [Method] Forty--eight fluconazole-susceptible stains and 10 fluconazole-resistant stains of C. albicans were analyzed, with two resistant stains induced in vitro. All of them were genotyped by arbitrary primed polymerase chain reaction (AP-PCR) fingerprinnting employing one interrepeat primes (1251 primers). The image was captured using computer-assisted system with Labwork 4.0 software to analyze the gel patterns of all isolates. The data were processed by SPSS 11.5 statistical software.[Results] Primer 1251 was suitable for fmgerprinting analysis, and was found to generate the reproducible fingerprinting profiles, yielding well-resolved banding patterns. The AP-PCR profiles of 8 resistant stains isolated clinically were highly similar according to similarity coefficient (71.7%). Furthermore, the similarity coefficient of 7 resistant isolates (86.6%) was significantly higher than that of total 58 stains (47.3%). For two induced isolates, their genotypes did not change much after resistance-inducement in vitro. [Conclusions] No specific DNA profiles of C.albic and indicating were identified. However, our results demonstrated a strong genetic correlation between fluconazole-resistant patterns and its APPCR profiles in C.albicans.
出处
《中山大学学报(医学科学版)》
CAS
CSCD
北大核心
2007年第1期35-39,共5页
Journal of Sun Yat-Sen University:Medical Sciences