IL-18在系统性红斑狼疮肾损害患者外周血中过度表达

Overexpression of Interleukin-18 in Systemic Lupus Erythematosus Patients with Renal Damage

【目的】探讨IL-18在系统性红斑狼(SLE)肾损害发生发展中的作用。【方法】采用RT-PCR及ELISA法测定了20例SLE患者(其中10例伴有肾损、10例无肾损)及10例健康人的外周血单个核细胞(PBMC)表达IL-18 mRNA和血清IL-18蛋白水平。【结果】SLE肾损组和非肾损组、健康对照组PBMC的IL-18 mRNA表达分别为1.35±0.30、0.91±0.27、0.69±0.17。SLE肾损组和非肾损组、健康对照组血清IL-18蛋白水平分别为:(799±237)pg/mL、(163±53)pg/mL、(114±39)pg/mL。SLE肾损患者血清IL-18蛋白、PBMC的IL-18 mRNA平均表达水平较健康人、非肾损SLE患者显著增高;SLE非肾损患者血清IL-18蛋白、PBMC的IL-18 mRNA表达水平也高于健康对照组,差异有统计学意义(P﹤0.05)。【结论】IL-18的过度表达可能在SLE特别是狼疮性肾损害过程中起着一定作用。

[Objective] To study the crucial role of IL-18 in systemic lupus erythematosus （SLE） patients with renal damage. [Methods] The levels of serum IL-18 and expression of IL-18 mRNA in peripheral blood mononuclear cell （PBMC） from 20 SLE patients （10 SLE patients with renal damage, 10 SLE patients without renal damage） and 10 healthy controls were evaluated by ELIZA and RT-PCR. [Results] The levels of IL-18 mRNA of PBMC from SLE patients with and without renal damage, healthy control were： 1.35±0.30, 0.91±0.27, and 0.69±0.17. The serum IL- 18 levels of SLE patients with and without renal damage, healthy control were： （799±237） pg/mL, （163±53） pg/mL and （114±39） pg/mL The levels of serum IL-18 and expression of IL-18 mRNA in PBMC from SLE with renal damage were several times of the levels in healthy controls and SLE without renal damage, compared with healthy controls, it also significantly increased in SLE patients without renal damage （respectively, P 〈 0.05）. [Conclusions] Increased expression and secretion of IL-18 in peripheral blood may play a role in the pathogenesis of SLE patients, especially SLE patients with renal damage.