可生物降解聚合物载体微球口服防龋疫苗免疫效果

Immune Effect of Biodegradable Polymer Microparticle Vaccine Against Dental Caries by Oral Administration

【目的】以PAc多肽(301～319)作为抗原,PBLG-PEG-PBLG做载体制备防龋微球疫苗,口服免疫SD大鼠,观察其对变链菌在大鼠牙面的黏附及对龋病发生的影响效果。【方法】28只出生30d的雄性SD大鼠随机分成4组建立龋模型,对各组大鼠口腔中变链菌的黏附菌落计数,根据Keyes评分标准做龋齿记分。【结果】PAc多肽抗原微球疫苗口服组的变形链球菌数为(13.2±8.16)×104CFU/ml;磨牙龋齿Keyes计分为31.8±6.77,均显著低于对照组(P<0.01)。【结论】口服PAc多肽抗原微球防龋疫苗均能减少龋模型大鼠龋齿的发生,以光滑面龋的减少更显著。

[Objective]The peptide derived from Pac （301-319） was selected as antigens. PBLG-PEG-PBLG block copolymer was selected as carrier to prepare microparticle vaccine which can hold PAc peptide （301-319）. Then we investigated the colonization of Streptococcus mutans on experimental rat tooth surface and the effect of oral administrated vaccine against rat dental caries. [ Methods ] Twenty-eight Spragne-Dawley gnotobiotie rat models were randomly divided into 4 groups. The colonization numlmrs of S. mutans were counted and the presence of caries was quantified by the Keyes scoring system. [ Results ] The bacteria numlmrs of the PAc peptide microparticle group were （13.2±8.16）×10^4 CFU/ml and the Keyes scores were 31.8±6.77. Both were significantly lower than those of the control group（P〈 0.01）. [Conclusions] The PAc peptide micropaticle vaccine by oral administration could reduce the development of experimental rat caries, especially the caries on tooth smooth surface.