摘要
SiO2 monoliths with double-pore structure were synthesized with tetraethyl orthosilicate, polyethylene glycol, starch, ethanol and ammonia as the main raw materials via a sol-gel route. The effect of different content of starch of the samples was studied on the mesopore structure, macropore appearance, hydrothermal stability and enzyme activity to immobilize gluczyme. The results indicate that porous blocks with mesopores of 10~12 nm and macropores of 10~30 μm can be formed by the removal of polyethylene glycol and starch after heating at 600 ℃. The minimal density of the obtained samples was 0.34 g·cm-3 and the maximal porosity was 76%. After being immersed in water at 80 ℃ for 7 d, the density, porosity and shape of N2 adsorption-desorption curves of the samples were almost unchanged, showing that the sample has a good hydrothermal stability. The initial enzyme activity of sample P2KD30 was 11 190 U, and definite enzyme activity could be maintained after five cycles.
SiO2 monoliths with double-pore structure were synthesized with tetraethyl orthosilicate, polyethylene glycol, starch, ethanol and ammonia as the main raw materials via a sol-gel route. The effect of different content of starch of the samples was studied on the mesopore structure, macropore appearance, hydrothermal stability and enzyme activity to immobilize gluczyme. The results indicate that porous blocks with mesopores of 10-12 nm and macropores of 10-30 I^m can be formed by the removal of polyethylene glycol and starch after heating at 600℃. The minimal density of the obtained samples was 0.34 g·cm^-3 and the maximal porosity was 76%. After being immersed in water at 80 ℃ for 7 d, the density, porosity and shape of N2 adsorption-desorption curves of the samples were almost unchanged, showing that the sample has a good hydrothermal stability. The initial enzyme activity of sample P2KD30 was 11 190 U, and definite enzyme activity could be maintained after five cycles.
出处
《无机化学学报》
SCIE
CAS
CSCD
北大核心
2007年第1期164-168,共5页
Chinese Journal of Inorganic Chemistry
基金
上海市科委科技攻关项目(No.0252NM006)资助
关键词
双孔结构
多孔块体
溶胶-凝胶法
酶载体
酶活性
double-pore structure
porous monolithic
sol-gel
enzyme carrier
enzyme activity