苦参碱对胃腺癌细胞SGC7901黏附和移动的影响

Inhibitive Effect of Matrine on Adhesion and Migration of SGC7901 Cells

目的:探讨不同浓度苦参碱对SGC7901细胞黏附和移动能力的影响。方法:5,50,100,250和500 mg/L苦参碱作用SGC7901细胞24 h后,黏附实验检测细胞黏附率,划痕损伤实验(Wound healing assay)检测迁移速度,在Transwell培养体系中,观察苦参碱对SGC7901细胞的抑制作用,PKA试剂盒检测PKA活性。结果:5,50,100,250和500 mg/L苦参碱处理24 h后,SGC7901细胞黏附率分别为78.56%,44.28%,42.48%,39.75%和31.54%,总体呈下降趋势,50 mg/L组与5 mg/L和500 mg/L组比较差异有显著性(P<0.01);与100 mg/L和250 mg/L组比较差异无显著性(P>0.05)。50 mg/L苦参碱作用24 h后SGC7901细胞迁移速度显著降低(P<0.01),Transwell培养体系中,苦参碱组上层迁移到下层的细胞明显少于不加苦参碱组(P<0.01),且PKA活性明显降低(P<0.01)。结论:苦参碱能抑制SGC7901细胞黏附和运动。

Objective： To explore the inhibitive effect of matrine on adhesion and migration of SGC7901 cells. Methods： Adhesive ratio was detected by adhesion assay and migration rate was measured by wound healing assay. A microchemotaxis chamber, Transwell system, was used to observe the effects of matrine on the migration of SGC7901, and PKA activity was tested by PKA activity assay. Results： After SGC7901 cells were treated with matrine of 5, 50, 100, 250 and 500 mg/L for 24 hours, adhesive ratios were 78.56%, 44.28%, 42. 48%, 39.75% and 31.54% respectively. Compared with that of the 5 mg/L and 500 mg/L groups, the adhesive ratio of 50 mg/L group was significantly different （P〈0.01）, but had no significant difference when compared with the 100 mg/L group and 250 mg/L group（P〉0.05）. After SGC7901 cells were treated with 50 mg/ L matrine for 24 hours, the migration rate was markedly reduced （P〈0.01）, and matrine significantly decreased SGC7901 migration across the membrane of the microchemotaxis chamber as compared with those in the control group （P〈0.01）, and PKA activity also was significantly reduced （P〈0.01）. Conclusion. Matrine can inhibit adhesion and migration of SGC7901 cells.