发光法分析莪术油等中药对肝、胃癌细胞增殖活性抑制作用的实验研究

Empirical Studies on the Effect of Aromatic Turmeric Oil for Inhibiting Proliferation of Hepatoma and Gastric Carcinoma Cells using Bioluminescence Assay

目的:探讨抗癌中药莪术油、华蟾素、龙葵碱与肝癌细胞株SMMC7721、胃癌细胞株NKM45增殖活性的抑制作用。方法:应用生物发光法(BLA法)测定莪术油、华蟾素和龙葵碱与SMMC7721、NKM45作用后的ATP含量,并与5-氟脲嘧啶(5-FU)的作用进行比较,分析莪术油对NKM45生长曲线的影响。结果:莪术油等三种中药与SMMC7721、NKM45作用后的ATP含量明显下降(P<0.05～P<0.01);与5-FU作用后的ATP含量比较,无明显差异(P>0.05)。莪术油对NKM45的存活率有明显的抑制作用。结论:莪术油等中药能够抑制SMMC7721、NKM45细胞的增殖活性。BLA法可广泛应用于抗肿瘤药物的筛选。

Objective： To explore the anti-proliferative effects of anti-cancer Chinese medicinal materials such as aromatic turmeric oil on hepatoma （SMMC7721） and gastric carcinoma cells （NKM45）. Methods： Bioluminescence assay （BLA） was applied to determine the ATP concentration when SMMC7721 and NKM45 were exposed to aromatic turmeric oil, cinobufacin and solanine. The effect of aromatic turmeric oil on the growth curve of NKM45 was also analyzed. Results： The concentration of ATP was significantly decreased when the tumor cells were exposed to 5-Fluorouracil （5-FU）. It was also was significantly decreased when SMMC7721 and NKM45 were treated with cinobufacin, solanine, or aromatic turmeric oil P〈0.05-P〈0.01）. There was no significant difference in response between the tumor cells that were treated with the Chinese medicinal materials and those exposed to 5-FU （P〉0.05）. The aromatic turmeric oil significantly inhibited the survival rate of NKM45. Conclusion： Aromatic turmeric oil, cinobufacin, and solanine can inhibit the proliferative activity of SMMC7721 and NKM45. BLA assay can be used to screen anti-tumor drugs.