金属硫蛋白-1，2基因在燃煤型砷中毒患者中的表达

Expression of MT-1 and MT-2 genes in blood cells in coal-burning arsenism

目的观察金属硫蛋白-1，2（MT-1，2）基因在燃煤型砷中毒患者外周血细胞中的表达。方法选取燃煤型砷中毒患者及非病区正常对照人群各50例，采用实时荧光定量RT—PCR技术测定外周血细胞中MT-1A、MT-1H、MT-2A mRNA的表达。结果与对照组比较，砷中毒组患者外周血细胞MT-1A、MT-1H、MT-2A基因mRNA表达均明显降低，差异有统计学意义（t=33．40、45．64、24．53，P〈0．01）。结论MT在燃煤型砷中毒代谢中有重要的意义，MT mRNA表达降低可能是砷毒性和致癌性的一个易感因素。

Objective To elucidate the molecular mechanism of arsenism caused by coal-burning and to investigate metallothionein （MT-1A, MT-1H, MT-2A） expression in blood cells in coal-burnting arsenism. Methods Blood cell samples were collected from arsenic-exposing patients and health subjects in Guizhou and total RNA was extracted and purified. The expression of three human MT isoforms was determined with real-time quantitative RT- PCR. The β-actin was used as quality control in the real-time quantitative RT-PCR. Results The relative expression of MT-1A, MT-1H and MT-2A was lower in arsenic exposed patients as compared to health subjects controls （t = 33.40,45.64,24.53,P 〈 0.01）. Conclusions The MT is of important significance in the metabolism of coal-burning arsenism. The suppression of MT could be a susceptible factor in arsenic toxicity and arsenic carcinogenesis.