摘要
用新鲜的人绒毛膜组织，抽提组织总ＲＮＡ并通过逆转录反应合成ｃＤＮＡ第一链。利用人工合成的一对寡核苷酸引物，采用ＰＣＲ技术特异性地扩增ｈＣＧ－β－ｃＤＮＡ。琼脂糖凝胶电泳结果显示扩增片段大小为５００ｂｐ，同预计的片段长度相符。将此片段利用Ｔ－Ａ载体克隆至ＰＣＲⅡ质粒上并进行全序列分析，结果显示克隆片段包含ｈＣＧ－βｃＤＮＡ５’端和３’端非编码区及完整的编码区。
Total RNA was extracted from fresh human chorial tissue and then reversetranscripted into cDNA. We designed a pair of oligonucleotide primers to specifically amplify hCG-βcDNA.Electrophoresis in agarose gels indicates that the amplified product is about 500 bp as expected. The amplified product was cloned into PCR Ⅱ vector of TA cloning kit(Invetrogen, Ca, USA) and then sequenced.the sequencing analysis shows that the cloned fragment contains part of 5'and 3'non-coding region and the entire coding region of hCG-β.
