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RNA干扰及其植物抗病毒应用 被引量：15

RNA Interference and its Application in Plant Anti-virus
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摘要 【研究目的】RNA干扰(RNAinterference,RNAi)是分子生物学研究领域的一项新兴技术,在动植物的遗传改良、功能基因组、基因治疗等研究中广泛应用;【方法】它是指一些小的双链RNA分子介导的、序列特异的转录后基因沉默现象;【结果】本文就RNA干扰的作用机制、RNA干扰与植物病毒间的互作以及RNA干扰表达载体构建的一些研究进展进行了综述;【结论】随着RNAi研究的进一步深入,RNAi必将成为遗传育种工作者培育抗病毒植物的有力手段,促进植物抗病育种的发展。 [Objective] RNA interference is a new technique in molecular biological research. It had been widely used in the research of animal and plant for genetic improvement, functional genome and gene therapy. [Method] RNAi is a post-transcriptional gene-silencing phenomenon induced by double-stranded RNA. [Results] In this paper, the mechanism of RNA interference, the interaction of RNA interference and plant virus and the construction of RNAi expression vector were reviewed. [Conclusion] With further study on it, RNAi will become a powerful skill in plant anti-virus breeding.
作者 张恭 刘立峰 马峙英
机构地区 河北农业大学农学院
出处 《中国农学通报》 CSCD 2007年第1期42-45,共4页 Chinese Agricultural Science Bulletin
基金 河北省自然科学基金资助项目" 应用RNA干扰技术防治水稻条纹叶枯病研究"(C2006000447)
关键词 RNA干扰 植物抗病毒 表达载体 RNA interference, Plant anti-virus, Expression vector
分类号 Q752 [生物学—分子生物学]
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参考文献22

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二级参考文献128

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中国农学通报
2007年 第1期

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