摘要
在实验室内对桔全爪螨进行辛硫磷抗性选育,选育12代后抗性上升了18.6倍。比较抗辛硫磷品系和相对敏感品系体内多功能氧化酶的酶学特性及辛硫磷和增效醚(PBO)对该酶的抑制作用,结果表明,桔全爪螨抗性品系多功能氧化酶的活性、比活力和米氏常数(Km)均显著高于敏感品系。辛硫磷对敏感品系体内MFO的抑制效果显著强于抗性品系,且MFO活性抑制率与药剂抑制浓度之间呈显著的线性关系。以PBO作用于桔全爪螨,相对敏感品系和抗性品系MFO的活力均受到抑制,但对抗性品系的抑制效果强于敏感品系。研究结果说明桔全爪螨对辛硫磷的抗性形成与多功能氧化酶的活力的升高有关。
The resistance to Phoxim in Panonychus citri was selected in laboratory. After 12 generations of selections, Panonychus citri have developed 18.6-fold resistance to phoxim. By the Comparison of the properties of mixed function oxidase （MFO） between the resistant and susceptible strains and the inhibition of phoxim and PBO to MFO of P. citri, it was found that the activities, specific activity and the Michaelis- Menten constant （m） of MFO in the resistant strain were significantly higher than those in susceptible strain, and the inhibition of phoxim to MFO in susceptible strain was significantly greater than that in resistant strain. There was a significantly linear relationship between the inhibition rate of MFO activity and the inhibition concentration of the Phoxim. However, the inhibition of PBO to MFO in resistant strain was significantly higher than that in susceptible strain. It was suggested that the development of resistance to phoxim in P. citri might be related to the rise of the MFO activity.
出处
《中国农学通报》
CSCD
2007年第1期258-261,共4页
Chinese Agricultural Science Bulletin
基金
教育部新世纪优秀人才支持计划(NCET-04-0854)
重庆市科技创新能力建设"虫螨对环境胁迫适应的分子生态机理及适合度评估"(CSTC
2005CA1002)。
