气液环境下白腐真菌Phanerochaete chrysosporium在载体表面的附着性能研究

Adherence performance of white rot fungus phanerochaete chrysosporium to the support materials in gas and liquid phases

以白腐真菌Phanerochaetechrysosporium为研究对象,考察了该菌种在液相和气相环境中在陶粒、颗粒炭、玻璃珠、竹子、聚丙稀多面空心球、沸石上的附着和生长情况.研究结果表明,在液相环境中,白腐真菌Phanerochaetechrysosporium对竹子、沸石、陶粒、聚丙稀多面空心球有较好的附着能力,但菌体的生长量和形态特征受载体的影响而产生明显差异.在有玻璃珠和颗粒炭存在的液相环境中,均出现一定数量的菌丝小球,但在载体表面无附着现象.将附有菌体的载体置于气相环境中,发现白腐真菌在载体表面能够形成气生菌丝层.同时,显微镜下观察发现,菌体在沸石、陶粒和多面空心球表面呈绒毛状,质地松散,在竹子表面的菌体有明显的交织结构.

The adherence performance of white rot fungus Phanenochaete chrysosporium to different support materials was studied by batch tests. The selected support materials were ceramisite agitator, granular activated carbon, glass beads, bamboo, polypropylene spheres and zeolite. The results indicated that Phanerochaete chrysosporium could adhere well to the surfaces of bamboo, zeolite, ceramic ball and polypropyhne sphere in liquid phase. The amount of attached biomass and morphology of Phanerochaete chrysosporium showed great difference due to different support materials. Phanerochaete chrysosporium did not adhere on the glass beads and granular activated carbon, while some litde hypha ball formed in the liquid, After the support materials with attached biomass were exposed in gas phase, mycelial cushions were observed to form on the support materials and showed different typical style.