玻璃化法超低温保存石楠茎尖的初步研究

Cryopreservation of Photinia serrulata Shoot-Tips by Vitrification

A procedure was studied on cryopreservation of Photinia serrulata shoot-tips in vitro by vitrification. The excised shoot-tips which were cultured for 30 d, 1.5～2.0 mm long, were loaded with 60% PVS2 for 40 min at room temperature in effendoff tube, then were exposed to PVS2 at 0 ℃ for 50 min. Followed by changing the solution with fresh PVS2, the tubes were immersed into LN2 directly. After 24 h storage, the effendoff tubes were rapidly thawed in the water bath at 40 ℃, the shoot-tips were washed twice with 1.2 mol·L -1 sucrose solution for 10 min and transferred onto MS medium supplemented with 6-BA 2.0 mg·mL -1 . The cultures were kept in dark for one week prior to exposure to the light. Survival rate of shoot-tips was 93.33% by TTC examination, and regeneration rate reached 41.67%.

A procedure was studied on cryopreservation of Photinia serrulata shoot-tips in vitro by vitrification. The excised shoot-tips which were cultured for 30 d, 1.5 - 2.0 mm long, were loaded with 60% PVS2 for 40 min at room temperature in effendoff tube, then were exposed to PVS2 at 0 ℃ for 50 min. Followed by changing the solution with fresh PVS2, the tubes were immersed into LN2 directly. After 24 h storage, the effendoff tubes were rapidly thawed in the water bath at 40 ℃, the shoot-tips were washed twice with 1.2 mol· L^-1 sucrose solution for 10 min and transferred onto MS medium supplemented with 6-BA 2.0 mg· L^-1. The cultures were kept in dark for one week prior to exposure to the light. Survival rate of shoot-tips was 93.33 % by TTC examination, and regeneration rate reached 41.67%.