姜黄素预防大鼠肝纤维化效果的观察

Prophylactic Effect of Curcumin on Rats Hepatic Fibrosis

目的:明确姜黄素对实验性肝纤维化的预防效果。方法:以四氯化碳制作大鼠肝纤维化模型,同时按每100 g体重分别给予20 mg、10 mg、5 mg姜黄素灌胃处理,设立正常组、肝纤维化组和阳性对照组;8周后处死大鼠,取血检测血清ALT、AST、HA、PC-Ⅲ、TGF-β、TNF-α水平;肝脏组织HE及Masson染色观察病理改变,根据SSS计分系统评估肝纤维化程度。结果:姜黄素可以减少肝纤维化大鼠血清中明显升高的ALT、AST、HA、PC-Ⅲ、TGF-β、TNF-α水平,明显减轻肝纤维化程度,SSS计分在姜黄素组明显降低,接近正常(P<0.05),以上作用均随着姜黄素用量增大而加强。结论:姜黄素可以预防四氯化碳所致的大鼠肝纤维化,改善肝功能,具有一定的量效关系。

Objective： To observe prophylactic effect of curcumin on rat hepatic fibrosis induced by tetrachloride. Methods： Rat models of hepatic fibrosis were constructed hy intraperitoneally injection of tetrachloride. Curcumin of 20 mg, 10 mg,5 mg per 100 gram weight of rat were given to these rats respectively at the same time. Normal, fibrotic and positive groups were made as controls. After eight weeks, all rats were executed. Serum samples were kept for measuring serum levels of ALT,AST,HA,PC -Ⅲ ,TGF -β ,TNF -α. Left livers were extirpated for pathologic examination including H. E and Masson stainings. Grade of hepatic fibrosis were evaluate according to SSS system. Results：Serum levels of ALT,AST, HA, PC - Ⅲ ,TGF -β,TNF - α of fibrotic group inceased significantly compared with that of normal group, and serum level of these iteme were depressed obviously in curcumin group （ P 〈 0. 05）. Staining of H. E and Masson showed that degrees of hepatic fibrosis in curcumin groups were improved obviously compared with that of fibrotic group, and the scores of hepatic fibrosis in curcumin groups reduced significantly. Conclusion ： Curcumin Can prevent rat hepatic fibrosis and improve hepatic functions, and Its effect is in dose -dependent.