摘要
目的:探讨组织蛋白酶B(CB)特异性siRNA对人食管癌EC9706细胞体外侵袭力的抑制作用。方法:将体外转录合成的针对CB的特异性siRNA设10μmol/L、15μmol/L和20μmol/L3个不同剂量转染人食管癌EC9706细胞,用Boydenchamber体外侵袭实验检测转染前后细胞体外侵袭力的变化。对照组设无关siRNA转染组、空白对照组(转染空脂质体)及正常对照组(不进行任何操作)。结果:和正常对照组相比,特异性siRNA15μmol/L组和20μmol/L组穿越Matrigel胶的细胞数明显减少(P<0.05),而10μmol/L特异性siRNA组、空白对照组和无关siRNA转染组与正常对照组之间差异均无统计学意义(P>0.05)。结论:特异性CBsiRNA能有效抑制人食管癌EC9706细胞的体外侵袭力。
Aim : To investigate the inhibitory effect of specific siRNA of cathepsin B (CB) gene on the invasiveness of human esophageal carcinoma EC9706 cells. Methods: EC9706 cells were transfected with synthesized siRNA of CB gene, and the transfection concentration was 10 μmol/L, 15 μmol/L, and 20 μmol/L. The EC9706 cells transfected with non-specific siRNA, empty liposome, and untransfccted EC9706 cells were used as control. Boyden chamber experiment in vitro was used to detect the invasion ability of EC9706 cells. Results: Compared with normal control, the number of cells traversed Matrigel was decreased obviously in 15 μmol/L specific siRNA group and 20 μmol/L specific siRNA group( P .〈 0.05) ; there were no significant differences in the number of cells traversed Matrigel among 10 μmol/L specific siRNA group, empty liposome group, non-specific siRNA group and normal control group ( P 〉 0.05 ). Conclusion : Specific siRNA can effectively inhibit the invasiveness in vitro of human esophageal carcinoma EC9706 cells.
出处
《郑州大学学报(医学版)》
CAS
北大核心
2007年第1期17-19,共3页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省科技攻关基金资助项目0624410091