组织蛋白酶B特异性siRNA对人食管癌EC9706细胞体外侵袭力的抑制作用

Inhibitory effects of siRNA on the invasiveness in vitro of human esophageal carcinoma EC9706 cells

目的:探讨组织蛋白酶B(CB)特异性siRNA对人食管癌EC9706细胞体外侵袭力的抑制作用。方法:将体外转录合成的针对CB的特异性siRNA设10μmol/L、15μmol/L和20μmol/L3个不同剂量转染人食管癌EC9706细胞,用Boydenchamber体外侵袭实验检测转染前后细胞体外侵袭力的变化。对照组设无关siRNA转染组、空白对照组(转染空脂质体)及正常对照组(不进行任何操作)。结果:和正常对照组相比,特异性siRNA15μmol/L组和20μmol/L组穿越Matrigel胶的细胞数明显减少(P<0.05),而10μmol/L特异性siRNA组、空白对照组和无关siRNA转染组与正常对照组之间差异均无统计学意义(P>0.05)。结论:特异性CBsiRNA能有效抑制人食管癌EC9706细胞的体外侵袭力。

Aim ： To investigate the inhibitory effect of specific siRNA of cathepsin B （CB） gene on the invasiveness of human esophageal carcinoma EC9706 cells. Methods： EC9706 cells were transfected with synthesized siRNA of CB gene, and the transfection concentration was 10 μmol/L, 15 μmol/L, and 20 μmol/L. The EC9706 cells transfected with non-specific siRNA, empty liposome, and untransfccted EC9706 cells were used as control. Boyden chamber experiment in vitro was used to detect the invasion ability of EC9706 cells. Results： Compared with normal control, the number of cells traversed Matrigel was decreased obviously in 15 μmol/L specific siRNA group and 20 μmol/L specific siRNA group（ P .〈 0.05） ; there were no significant differences in the number of cells traversed Matrigel among 10 μmol/L specific siRNA group, empty liposome group, non-specific siRNA group and normal control group （ P 〉 0.05 ）. Conclusion ： Specific siRNA can effectively inhibit the invasiveness in vitro of human esophageal carcinoma EC9706 cells.