雄性无毛小鼠睾丸、附睾的发育及组织形态学观察

Observation of development and histological changes of male hairless mice testis and epididymis

目的：研究雄性无毛小鼠生殖器官的发育及组织形态学改变。方法：取8—10周龄雄性无毛小鼠5只，昆明种雄性小鼠10只。用天平称取动物体质量后，颈椎脱臼处死，摘取睾丸、附睾、前列腺，并用分析天平称重，根据每只小鼠体质量计算脏器指数。所有组织切片后行HE染色，在光学显微镜下观察睾丸曲细精管、生精细胞及间质细胞的形态和数量及附睾组织结构。结果：雄性无毛小鼠睾丸、附睾及前列腺脏器指数与昆明种小鼠相比差异无统计学意义。但睾丸组织学存在曲细精管细胞层次减少，粗线期初级精母细胞减少，胞核结构模糊。附睾及前列腺组织未见明显异常。结论：雄性无毛小鼠睾丸组织学改变可能是导致繁种、育种困难的原因之一。

Aim： To investigate the developmental and histological changes of reproductive organs in male hairless mice. Methods： Five male hairless mice and 10 Kunming mice aged 8 -10 weeks were chosen. The testis, epididymis, and prostate gland of mice were removed and weighted. Afterwards the tissue from these organs was fixed and stained with HE. Results： There was no significant difference between male hairless mice and Kunming mice in the organ index of testis, epididymis, and prostate gland, however, some histological changes were found in testis, including the decrease of cellular layer in convoluted seminiferous tubule, the unclarity of cell nucleus and the primary spermatoeytes reduced. No obvious morphological change in epididymis or prostate gland was found. Conclusion： The histological change in male hairless mice testis may be one of the factors responsible for its reproductive difficulty.