近交系小鼠微卫星位点的多态性观察

Microsatellite DNA polymorphisms in inbred strain mice

目的：观察不同品系小鼠微卫星位点的多态性。方法：取6-10周龄近交系BALB／C、豫医无毛小鼠、C57、CBA及DBA／2小鼠各5只，颈椎脱臼处死后取新鲜的脑组织0．3-0．5cm^3，提取DNA。随机选择位于小鼠2、3、4和16号染色体上的5对微卫星引物（D2Nds3、D3mit17、D3mit18、D4mit2、D16mit4），应用PCR技术对5种近交系小鼠的DNA多态性进行研究。结果：5对引物在近交系小鼠各基因座上均出现一清晰条带，D16mit4、D3mit17、D3mit18具有多态性，D4mit2、D2Nds3不具有多态性。结论：筛选出的引物D16mit4、D3mit17、D3mit18可用于检测近交系小鼠的品系来源和遗传背景，且所检测的小鼠符合近交要求。

Aim. To study the polymorphisms of microsatellite of inbred strain mice. Methods：Five microsatellites DNA loci on different chromosomes in five kinds of inbred strain mice including BALB/C, Yuyi hairless mice, CBA, C57 and DBA/2 were investigated by PCR analysis with five pairs of primers for D2Nds3, D3mit17, D3mit18, D4mit2, and D16mit4. Results： An allelic gene band was observed with five pairs of primers. Polymorphism was found at D16mit4, D3mit17, D3mit18 loci,but not at D4mit2 or D2Nds3 loci. Conclusion： The screened primers D16mit4, D3mit17, and D3mit18 could be used to detect the origin and genetic background of inbred strain mice.