卵巢上皮性肿瘤组织中EphA2和E-钙黏素测定

Expression of EphA2 and E-cadherin in epithelial ovarian cancer tissue

目的:探讨卵巢上皮性肿瘤组织中EphA2和E-钙黏素的表达及其与临床病理特性、预后的关系。方法:采用免疫组织化学SP法检测60例卵巢恶性上皮性肿瘤(浆液性乳头状囊腺癌36例,黏液性乳头状囊腺癌15例,子宫内膜样癌6例,透明细胞癌3例;FIGO分期Ⅰ期4例,Ⅱ期5例,Ⅲ期23例,Ⅳ28例;组织学分级G115例,G27例,G338例)、14例卵巢交界性上皮性肿瘤、25例卵巢良性上皮性肿瘤及10例正常卵巢组织中EphA2及E-钙黏素蛋白的表达情况。结果:在正常卵巢、卵巢上皮性良性肿瘤、卵巢交界性上皮性肿瘤和卵巢恶性上皮性肿瘤组织中,EphA2蛋白的阳性表达率分别为10.0%(1/10)、20.0%(5/25)、57.1%(8/14)、85.0%(51/60),差异有统计学意义(P<0.01);E-钙黏素蛋白的阳性表达率分别为20.0%(2/10)、88.0%(22/25)、71.4%(10/14)、36.7%(22/60),差异有统计学意义(P<0.001)。随着卵巢肿瘤恶性程度的增高,EphA2的阳性表达率呈递增趋势,E-钙黏素的阳性表达率呈递减趋势,2者呈负相关(r=-0.396)。EphA2和E-钙黏素的蛋白过表达均与卵巢恶性上皮性肿瘤FIGO分期、组织学分级及有无腹膜转移密切相关。结论:在卵巢恶性肿瘤的发生发展过程中EphA2起一定的作用,其过表达可导致细胞的恶性转化。E-钙黏素与EphA2的联合检测可作为评价卵巢恶性上皮性肿瘤患者生存及预后的指标。

Aim： To investigate the relationship of EphA2 expression with E-cadhcrin, clinical pathological character and prognosis in epithelial ovarian cancer tissue. Methods：The positive expression rates of EphA2 and E-cadherin in 60 cases of malignant epithelial ovarian cancer, 14 cases of borderline epithelial cancer, 25 cases of benign epithelial ovarian tumor and 10 cases of normal ovary tissue were detected using immunohistochemisty. Results ： The positive rates of EphA2 protein in normal ovary, benign epithelial ovarian cancer, borderline epithelial cancer, and malignant epithelial ovarian cancer tissue were 10.0% （ 1/10）, 20.0% （5/25）, 57.1% （8/14） , and 85, 0% （ 51/60）, respectively, and there were statistical differences （ P 〈 0. 01 ）. The positive rates of E-cadherin protein in the above tissue were 20.0% （ 2/10）, 88.0% （22/25） ,71.4% （10/14） ,36.7% （22/60）, respectively, and there were statistical differences （P 〈0. 001 ）. With the increase of the malignant degree of ovarian carcinoma, the positive rates of EphA2 increased while that of E-cadherin decreased. The expression of EphA2 was negatively correlated with that of E-cadherin（ r = -0. 396）. The expression level of EphA2 and E-cadherin was correlated with clinical stage, histological grade and metastasis of peritoneum. Conclusion ： E- phA2 plays an important role in the progression and metastasis of epithelial ovarian cancer. The overexpression of EphA2 may induce cell canceration. Detection of EphA2 and E-cadherin may be used as the indexes of the survival and prognosis for patients with epithelial ovarian cancer.