乳癌组织中脆性组氨酸三联体蛋白检测

Expression of fragile histidine triad in breast carcinoma tissue

目的:检测乳癌组织中脆性组氨酸三联体(FHIT)蛋白的表达。方法:应用免疫组织化学SP法,检测53例乳癌组织(Ⅰ期6例,Ⅱ期13例,Ⅲ期25例,Ⅳ期9例;有淋巴结转移38例,无淋巴结转移15例;高中分化31例,低分化22例)及14例癌旁正常组织中FHIT的表达。结果:①乳癌组织中FHIT蛋白阳性表达率(49.1%,26/53)明显低于正常乳腺组织(92.9%,13/14),P<0.05。②53例乳癌组织中,中高分化组与低分化组间(54.8%νs40.9%),淋巴结转移组与无淋巴结转移组间(44.7%νs60.0%),Ⅰ+Ⅱ期组与Ⅲ+Ⅳ期组间(57.9%νs44.1%)FHIT蛋白阳性表达率差异均有统计学意义(P均<0.05)。结论:FHIT基因与乳癌的发生、发展有关,可作为判定乳癌发生及转移能力的一项客观指标。

Aim： To investigate the expression of fragile histidine triad（FHIT） in breast carcinoma tissue. Methods： The expression of FHIT was detected using immunohistochemieal SP method in 53 cases of breast carcinoma, and 14 cases of adjacent normal breast tissue. Results： The positive rate of FHIT in breast carcinoma tissue and normal breast tissue was 49.1% and 92.9% , respectively, and there was significant difference （P 〈 0.05 ）. The positive rates of FHIT in high and middle-differentiated tissue were significantly higher than that in low differentiated tissue （54.8% vs 40.9% ）. Tumors with lymph node metastases （44.7%） had lower FHIT protein expression than those without metastasis （60.0%） （P 〈 0.05 ）. There was significant difference in FHIT expression between stage Ⅰ ＋ Ⅱand stage Ⅲ＋ Ⅳ（57.9% vs 44.1% ） （ P 〈 0.05 ）. Conclusion ： FHIT gene plays an important role in the occurrence and development of breast carcinoma, it is suggested that FHIT can be a useful marker for predicting invasion and metastasis ability of breast carcinoma.