绿麦隆和阿特拉津联合染毒对小鼠睾丸的影响

Combined Effect of Atrazine and Chlorotoluron on Mice Testis,a Morphological Research

目的研究绿麦隆和阿特拉津单体系及联合作用对小鼠睾丸形态及结构的影响。方法将昆明种小鼠按灌胃农药和剂量随机分为20组犤1个对照组,4个绿麦隆染毒组(321.5、1250、2500、5000mg/kg),3个阿特拉津染毒组(218.75、875、1750mg/kg),12个联合染毒组犦,每组10只。连续25d经口灌胃,每天1次,每次1ml。并用光学显微镜、电子显微镜对小鼠睾丸病理学变化进行观察。结果农药单体系及联合染毒体系各剂量组对小鼠睾丸均有不同程度的损伤。与对照组比较,光学显微镜观察所见为各剂量组出现不同程度生精上皮细胞排列疏松、紊乱,生精细胞脱落,层次减少,病变严重;电子显微镜观察为各剂量组出现不同程度生精细胞线粒体呈空泡样改变,核膜肿胀、弯曲,支持细胞功能低下随染毒剂量增加,上述病理学变化有加重趋势,联合染毒组病理变化比单体系染毒组更明显。结论绿麦隆和阿特拉津对小鼠睾丸的毒性与染毒剂量相关,联合作用体系加重了对小鼠睾丸的毒性效应。

Objective To study the combined effect of chlorotoluron and atrazine on the testis of mice. Methods Kunming mice were divided into groups and treated with chlorotoluron and atrazine alone or in combination by garage at different doses for 25 consecutive days. Microscope and electron microscope were used to observe the morphological changes. Results The herbicides used alone or in combination, at all the test doses, caused the morphological changes in degrees in the testis. Under the light microscope, seminiferous epithelium arrayed loosely and disorderedly, spermatogenic cell shed, and layers lessened compared with the control group. Under the electron microscope, mitochondrla in the seminiferous epithelium appeared vacuolated, karyotheca swelled, bent and the function of sustentacular cell declined. Compared with alone use groups, the pathological changes were more serious in combination use groups. Conclusion Chlorotoluron and atrazine can produce a combined toxic effect on the testis of mice.