摘要
目的探讨心肌梗死(AM I)后心肌组织中单核细胞趋化蛋白-1(M CP-1)及基质细胞衍生因子-1(SDF-1)的表达及其意义。方法以原位杂交及免疫组化法检测AM I大鼠发病1、2、4、7、14、28、56 d后梗死心肌及非心肌梗死大鼠心肌中SDF-1、M CP-1的表达情况。结果梗死中心区M CP-1于心肌梗死后第1 d升高,第2天达峰值,至第14天渐降至正常;梗死周围区M CP-1心肌梗死后第1天升高,第7天达峰值,至第28天恢复正常;梗死中心区及周围区SDF-1均于心肌梗死后第1天升高并达峰值,至第14天渐恢复至正常;非梗死心肌M CP-1、SDF-1的表达在正常范围,且不随时间改变而变化。结论AM I后早期梗死中心组织及周围区域SDF-1、M CP-1表达水平升高;根据梗死中心区SDF-1、M CP-1表达情况选择治疗时间窗可提高治疗效果,有利于梗死心肌修复。
[Objective] To investigate the expression of SDF-1 and MCP-1 in myocardial infarct tissue. [Methods] The expression of monocyte chemotactic protein-1 （MCP-1） and stromal cell-1 derived factor-1 （SDF- 1） were measured by in situ hybridization and immunohistochemistry in sham operated or infarcted hearts at 1, 2, 4, 7, 14, 28 and 56 days post operation. [Results] Expression of MCP-1 increased at the first day, peaked at 2nd day and decreased there after in the center of myocardial infarct tissue, peaked at 7th day and decresed thereafter in peri-myocardial infarct tissue. Expression of SDF-1 increased and peaked at the first day and decreased thereafter in the center of myocardial infarct atissue and peri-myocardial infarct stissue. Expression of MCP-1 and SDF-1 in tissue without myocardial infarct were normal. [Conclusions] We may treat myocardial infarct according to the expression of MCP-1 and SDF-1.
出处
《山东医药》
CAS
北大核心
2007年第2期7-10,共4页
Shandong Medical Journal
基金
南京市医学重点科技发展项目(ZKX05005)。
