ATP后处理对兔缺血再灌注心肌细胞凋亡及NF-κB表达的影响

Effects of ATP postconditioning on apoptosis and NF-κB expression of cardiomyocytes with ischemic reperfusion

目的观察三磷酸腺苷(ATP)后处理对兔缺血再灌注心肌细胞凋亡和核因子-κB(NF-κB)表达的影响。方法将64只雄性大耳白兔随机分为四组,每组16只。对照组:结扎冠状动脉前降支40 m in,再灌注180m in。缺血预处理组:3次短暂结扎冠状动脉前降支(5 m in闭塞、10 m in再灌注,重复3次),其余同对照组。缺血后处理组:方法同对照组,仅于再灌注初期结扎30 s、再灌注30 s,反复3次形成缺血后处理。ATP后处理组:方法同对照组,仅于再灌注初期自耳缘静脉泵入ATP 4 m g/kg,20 m in内滴完。应用免疫组化法测定组织中NF-κB的表达,应用TUNEL技术测定组织中细胞凋亡率,测定心肌梗死面积。结果与对照组比较,缺血预处理组、缺血后处理组和ATP后处理组细胞凋亡率明显减低,心肌梗死面积明显减小,NF-κB表达降低。结论ATP后处理对缺血再灌注损伤的心肌有保护作用,可能机制是抑制NF-κB参与的细胞凋亡。

[Objective] To evaluate the cardioprotective effects offered by adenosine triphosphate （ATP）,and discuss it＇s protective mechanism. [Methods] In anesthetized open-chest rabbits, the left anterior descending artery （LAD） was occluded for 40 mins and reperfused for 3 hours. In control group （n=16）, there was no intervention. In preconditioning group （n= 16）, the LAD was occluded for 5 mins and reperfused for 10 mins, and repeated this process for three times before the prolonged occluded. In postconditioning group （n= 16）, at the start of reperfusion, three cycles of 30-s reperfusion and 30-s LAD reocclusion preceded the 3 hours of reperfusion. In ATP group （n=16）, at the start of reperfusion, ATP （4 mg/kg） was injected within 30 mins. [Results] The expression of NF-κB and apoptosis rate in the control group was significantly higher than that of other groups （P〈0. 01）. Infarct size was significantly smaller in the preconditioning group, postconditioning group and ATP group （P〈0. 01）, compared with control group. [Conclusions] ATP has a good cardioprotective effects for cardiomyocytes with ischemic reperfusion, which partly due to the redusing of apoptosis.