PHBV共混物纤维制备组织工程支架的研究

Study on the Tissue Engineering Scaffold Prepared by PHBV Composites Fiber

利用聚羟基3-酸脂／聚羟基戊酸酯（PHBV）共混材料为基质，通过熔融纺丝、压片成型以及纤维熔结工艺，制得了组织工程用三维多孔支架。研究了PHBV其混材料的吸水半与溶胀比以及支架的熔结温度。结果表明：PHBV共混材料的吸水平较PHBV大为提高，有利于改善PHBV材料的亲水性。其溶胀比较低有助于保持组织工程爻架的尺寸稳定性。PHBV共混物纤维的最佳熔结温度在130-140℃范围．采用压片成型／纤维熔结法可制得孔径在300—500μm之间、贯通性好的三维立体支架。降解实验表明：支架材料的降解会引起pH值的微弱下降，支架材料的降解速率较慢。

In this paper, poly（3-hydroxybutyric acid-co-3-hydroxyvaleric acid） （PHBV） composites were used to prepare three dimensional scaffolds for tissue engineering after melt-spinning, molding and fiber bonding. Surface characteristics, pore sizes and their distribution and the bonding temperature of the scaffold were investigated by optical microscope （OM）, scanning electron microscopy （SEM） and differential scanning calorimeter （DSC）, respectively. The percent water absorption and dilatability were studied as well. However, the dilatability was very low which will contribute to the dimensional stability. The optimum bonding temperature should be from 13-140℃ The pore sizes range from 300-500μm. The scaffold exhibited 3D, connected network macro structures. The degradation of the fiber would cause a tiny decline of the pH value and the weight of the matrix was almost unchanged for a period of 10 weeks. All the results indicated the novel 3D macro porous scaffold was suitable for tissue engineering.