有龋和无龋儿童四种唾液蛋白成分的比较

Comparison of salivary proteins between children with early childhood caries and children without caries

目的比较3～4岁有龋儿童和无龋儿童非刺激性和刺激性唾液中 IgA、乳酸脱氢酶、溶菌酶和碱性磷酸酶含量的差异。方法分别采集98例有龋儿童和94名无龋儿童非刺激性和刺激性唾液,测定上述各成分含量。结果非刺激性和刺激性唾液中 IgA、乳酸脱氢酶和溶菌酶含量有龋组均显著高于无龋组(P<0.01),非刺激性唾液中碱性磷酸酶含量有龋组显著高于无龋组(P<0.05);但刺激性唾液中碱性磷酸酶含量两组问差异无统计学意义(P>0.05)。结论非刺激性和刺激性唾液中 IgA、乳酸脱氢酶和溶菌酶升高可能与婴幼儿龋的发生、发展有关。

Objective To compare the concentrations of IgA, lactate dehydrogenase, lysozyme and alkaline phosphatase （ALP） in unstimulated （UWS） and stimulated （SWS） whole saliva between children with severe early childhood caries （S-ECC） and children without caries. Methods One hundred and ninety-two children aged from 42 to 54 months were recruited from 11 urban kindergartens in Beijing. The S-ECC group contained 98 children with more than 5 decayed teeth, and the control group contained 94 cariesfree children. The age and sex were matched in the two groups. Two milliliter UWS and 2 ml SWS was collected between 9 and 11 a.m. The salivary IgA was measured by immunoturbidimetric technique. The concentrations of lactate dehydrogenase and ALP were measured by continuous monitoring method, while lysozyme was detected by turbidimetric technique. All results for paired observations between unstimulated and stimulated whole saliva were analysed by paired-samples t test. Results In beth UWS and SWS, the concentrations of IgA, lactate dehydrogenase and lysozyme in S-ECC children were higher than those in caries-free children （ P 〈 0. 01 ）, but the concentration of ALP showed no significant difference in SWS between S-ECC children and caries-free children （P 〉 0. 05 ） . Conclusions The presence of early childhood caries may be associated with an increase of IgA, lactate dehydrogenase and lysozyme in unstimulated and stimulated whole saliva.