槟榔碱及尼古丁对口腔角质形成细胞端粒酶逆转录酶表达的影响

Effects of arecoline and nicotine on the expression of hTERT in oral keratinocytes

目的观察槟榔碱及尼古丁对人口腔角质形成细胞(keratinocyte,KC)端粒酶逆转录酶(human telomerase reverse transcriptase,hTERT)mRNA 及蛋白表达的影响。方法实验分为0.030、0.060、0.090g/L 槟榔碱组、相应浓度槟榔碱+0.025g/L 尼古丁组和空白对照组。采用 Western blot和 RT-PCR 法观察槟榔碱、尼古丁对 KC hTERT mRNA 及蛋白表达的影响。结果槟榔碱呈剂量依赖性增加 KC hTERT mRNA 及蛋白的表达,0.030、0.060、0.090g/L 槟榔碱组 hTERT mRNA 及蛋白表达均高于空白对照组(P<0.001),0.025 g/L 尼古丁能增加槟榔碱诱导的 KC hTERT mRNA 及蛋白的表达,对槟榔碱有协同作用。结论槟榔碱对 KC hTERT mRNA 与蛋白表达有明显的诱导作用,尼古丁对槟榔碱具有协同作用。槟榔碱和尼古丁诱导 KC hTERT 过表达可能在口腔黏膜下纤维化癌变过程中起重要作用。

Objective To investigate the effects of arecoline and nicotine on the expression of human telomerase reverse transcriptase （hTERT） mRNA and protein in cultured normal human oral keratinocytes（KC）. Methods The experiments were divided into arecoline group, arecoline/nicotine group and control group. The hTERT mRNA and protein expression of KC was examined by reverse transcription polymerase chain reaction（RT-PCR） and Western blot. Results Arecoline could induce the hTERT mRNA and protein expression of KC in a dose dependent manner , the hTERT mRNA and protein expression of KC was higher in 0. 030, 0. 060, 0. 090 g/L arecoline group than control group （ P 〈 0. 001 ） . Nicotine （0. 025 g/L） increased hTERT mRNA and protein expression of KC induced by arecoline. Conclusions Arecoline could increase the expression of hTERT mRNA and protein in oral keratinocytes. Nicotine had a synergistic effect on arecoline, hTERT over-expression induced by arecoline and nicotine may play an important role in the malignant transformation of oral submucous fibrosis.