全自动微生物鉴定药敏分析仪对临床相关细菌和酵母菌鉴定能力的评估

Evaluation of Vitek 2 Compact for identification of clinically relevant bacteria and yeasts

目的评估 Vitek 2 Compact 全自动微生物鉴定药敏分析仪对临床相关细菌和酵母菌的鉴定能力。方法选取本院185株临床菌株(革兰阳性菌69株,革兰阴性菌66株,酵母菌50株)和50株本实验室保存的参考菌株(革兰阳性菌和革兰阴性菌各25株)为评估菌株,采用 Vitek2 Compact GP 卡、GN 卡和 YST 卡进行鉴定,其结果与参考方法 API 鉴定结果进行对照,所有低分辨及属对种错鉴定菌株均进行重复鉴定。结果在93株革兰阳性菌中有85株(91.40%)得到正确鉴定,其中5株(5.38%)为低分辨鉴定,另有8株(8.60%)为属对种错鉴定,90%的革兰阳性菌的鉴定在7 h 以内完成。在91株革兰阴性菌中90株(98.90%)为正确鉴定,其中5株(4.4%)为低分辨鉴定,仅1株(1.1%)为属对种错鉴定,90%以上肠杆菌科菌的鉴定在5 h 以内完成,90%以上非发酵糖菌的鉴定则在10 h 内完成。在50株酵母菌中46株(92%)为正确鉴定,其中8株(16%)为低分辨鉴定,另有4株(8%)为属对种错鉴定,45株(90%)在18.25 h 完成鉴定,5株(10%)在18.50 h 完成鉴定。结论 Vitek 2 Compact 能够对临床相关细菌和酵母菌进行可信的鉴定,并能大幅缩短常规鉴定时问,是临床微生物实验室病原菌诊断的有力工具。

Objective To evaluate a new system, Vitek 2 Compact, for identification of bacteria and yeasts. Methods 185 clinical isolates of Peking Union Medical College Hospital, including 69 grampositive strains, 66 gram-negative strains and 50 yeasts, and 50 reference strains in our laboratory, including 25 gram-positive and gram-negative strains respectively, were studied. All the strains were identified by Vitek 2 Compact with GP, GN or YST identification cards. The API method was used as the reference method. Results Among the 93 gram-positive strains, 85 strains （91.40%） were correctly identified, including 5 low discrimination identified strains, and 8 strains （8. 60% ） were correctly identified to the genus level, but misidentified to the species level. About 90% of gram-positive strains were identified within 7 k Out of 91 gram-negative strains, 90 strains （98.90%） were correctly identified, with 5 low discrimination identified strains, only 1 strain （ 1.1% ） was correctly identified to the genus level, but misidentified to the species level. Above 90% of Enterobacteriaceae were identified within 5 h, and over 90% of nonfermenting bacteria were identified within 10 k In the 50 strains of yeasts, 46 strains （92%） were correctly identified, including 8 low discrimination identified strains, and 4 strains （8%） were correctly identified to the genus level, but misidentified to the species level. In all the yeasts, 45 strains （90%） were identified in 18.25 h, and another 5 strains （ 10% ） were identified in 18. 50 h. Conclusions As Vitek 2 Compact system can give us reliable identification results of clinically relevant bacteria and yeasts, together with its significant reduction of handling time, it will definitely become a powerfnl tool in clinical microbiology laboratory.