应用Westgard方法评价决定图判断生化检测系统性能的可接受性

Using westgard's method evaluation decision chart for judging method performance of routine biochemistry items on Roche Modular PPI testing system

目的应用 Westgard 方法评价决定图判断常规生化项目在罗氏 Modular PPI 检测系统上分析性能的可接受性。方法定量测定各个实验项目的变异系数和不准确度(偏倚),应用Westgard 方法评价决定图,将27个常规生化项目的变异系数和偏倚以图像形式表达,再与要求的允许总误差(TEa)作比较,判断检测系统的分析性能可否接受。结果在 Modular PPI 检测系统上,钙的日间变异系数为0.08 mmol/L,偏倚为0.06 mmol/L,分析性能为临界;肌酐(Cr)、尿素(Ur)、葡萄糖(Glu)、钠(Na^+)、氯(Cl^-)和磷(P)的日间变异系数分别为3.20%、2.13%、1.52%、0.89 mmol/L、1.10%和1.55%,偏倚分别为4.79%、0.96%、4.63%、0.80 mmol/L、1.74%和4.13%,分析性能为良好;其余20个项目的分析性能均为优秀。结论 Modular PPI 生化检测系统常规生化项目均具有较好的精密度和准确度,分析性能可以接受。应用 Westgard 方法评价决定图判断生化检测系统的分析性能,简便易行,适合临床实验室应用。

Objective To judge method pedormance of routine biochemistry parameters on Roche Modular PPI testing system by using westgard＇s method evaluation decision chart. Methods We assessed imprecision （CV） from internal quality control and inaccuracy （bias） from external quality control evaluation. Combined estimates of imprecision and inaccuracy by plotting imprecision as the x-coordinate and inaccuracy as the y-coordinate to locate an expected operating point of every item on the chart. By comparing this operating point with allowable total errors （ TEa）, we can decide whether the performance is acceptable or not. Resets In the 27 different parameters tested, imprecision and bias of calcium were 0. 08 mmol/L and 0. 06 mmol/L respectively, its performance was marginal. The imprecision of creatinine, urea, glucose, sodium, chloride and phosphorus were 3.20% ,2. 13%, 1.52% ,0. 89 mmol/L, 1.10% and 1.55%, the bias were 4. 79% ,0. 96%, 4. 63% ,0. 80 retool/L, 1.74% and 4. 13% respectively, their performance was good. All other 20 items were of excellence pedormanco. Conclusions Routine biochemistry parameters on Roche Modular PPI testing system possessed good precision and accuracy, and their performance were accoptable. To judge method performance of biochemistry testing system by using westganl＇s method evaluation decision chart was easy to do and suited for clinical laboratory.