抗60000 SSA和52000 SSA抗体与系统性红斑狼疮和干燥综合征相关性的研究

Correlation of anti-60 000 SSA antibody and anti-52 000 SSA antibody with systemic lupus erythematosus and Sj■gren's syndrome

目的了解抗60000 SSA(Sjogren syndrome Type A,SSA)抗体和抗52 000 SSA 抗体与系统性红斑狼疮(SLE)和干燥综合征(SS)的相关性。方法用免疫印迹(Western-blot)方法检测59例抗 SSA 抗体阳性患者血清,其中 SLE 44例,原发性 SS 15例。结果抗60000 SSA 抗体阳性率在SLE 及原发性 SS 间差异无统计学意义(P>0.05),但单独携带抗60000 SSA 抗体在 SLE 及原发性 SS间差异有统计学意义,SLE 患者单独抗60000 SSA 抗体阳性率为39.47%(15/38),原发性 SS 患者6.67%(1/15);抗52000 SSA 抗体阳性率在 SLE 及原发性 SS 间差异有统计学意义(P<0.05),原发性 SS 患者抗52000 SSA 抗体阳性率为93.33%(14/15),SLE 患者为60.53%(23/38)。抗60000和抗52000 SSA 抗体阳性率在继发性 SS 及原发性 SS 间差异均无统计学意义。结论原发性 SS 患者以抗52000 SSA 抗体为主,单独出现抗60000 SSA 抗体的几率极小。单独抗52000 SSA 抗体阳性可能与原发性 SS 有关,而不利于继发性 SS 的诊断。单独携带抗60000SSA 抗体则以 SLE 为主。

Objective To investigate the correlation of anti-60 000 SSA antibody and anti-52 000 SSA antibody with systemic lupus erythematosus （SLE） and Sjioegren＇s syndrome （SS）. Methods Western blotting against purified 60 000 SSA antigenic polypeptide and 52 000 SSA antigenic polypeptide were done to detect the anti-60 000 SSA and anti-52 000 SSA antibodies in 59 serum samples positive in anti-SSA antibodies, among which 44 samples were from SLE patients and 15 samples from SS patients. Result There was no significant difference in positive rate of anti-60 000 SSA antibodies between the SLE and pSS patients （ P 〉 0.05 ）. But the sole positive rate of anti-60 000 SSA antibodies of the SLA patients was 39.47% （ 15/38）, significantly higher than that of the primary SS （pSS） patients（ （ 6.67%, 1/15, P 〈 0.05）. The positive rate of anti-52 000 SSA antibodies of the pSS patients was 93. 33% （14/15）, significantly higher than that of the SLE patients （23/38,60.53% , P 〈0.05）. There was no significant differences in positive rates of anti-60 000 SSA antibodies and anti-52 000 SSA antibodies between the secondary SS （sSS） and pSS patients （ P 〉 0.05 ）. Conclusion The pSS patients mainly present anti-52 000 antibodies and with a very low sole anti-60 000 SSA antibody positive rate. Sole positivity of anti- 52000 SSA antibodies maybe correlated with pSS and is not a strong implication for the diagnosis of sSS. Sole positive anti-60000 SSA antibodies rate can be seen maily in SLE.