细粒棘球绦虫重组BCG-Eg95疫苗免疫小鼠后IgG及其亚类和IgE的动态观察

Dynamic observation on IgG,its subclass and IgE in sera of mice immunized by recombinant BCG-Eg95 vaccine of Echinococcus granulosus

目的:动态观察细粒棘球绦虫重组BCG-Eg95疫苗免疫小鼠后IgG及其亚类和IgE的变化。方法:将疫苗分别采用鼻腔内接种和口服灌胃免疫BALB/C鼠,在免疫后0、2、4、6、8、10、12、14、16和18周各剖杀4只小鼠,眼球取血,常规ELISA测定血清中IgG及其亚类和IgE水平,同时设有PBS对照。结果:鼻腔内接种组的血清IgG、IgG2a和IgG2b水平均在免疫后2￣18周升高,分别在免疫后10、4和6周达最高水平;血清IgG1、IgG3和IgE水平均在免疫后2￣18周降低,分别在免疫后8、12和10￣12周达最低水平。口服免疫组的血清IgG、IgG2a和IgG2b水平均在免疫后2￣18周增加,分别在免疫后14、8和8周达峰值;血清IgG1、IgG3和IgE水平均在免疫后2￣18周下降,分别在免疫后8、6和16￣18周达最低水平。结论:细粒棘球绦虫重组BCG-Eg95疫苗在免疫早期即可诱导小鼠产生一个TH1型保护性免疫反应。

Objective：To dynamically observe changes of IgG,its subclass and IgE in sera of mice immunized by recombinant BCG-Eg95 vaccine of Echinocoecus granulosus （Eg）.Methods：BALB/C mice were intranasally or orally vaccinated and killed on 0、2、4、6、8、10、12、14、16 and 18w of immunization,with 4 in each killing.Sera were gathered from the eyeball to measure IgG, its subclass and IgE by routine ELISA,with PBS as control.Results：In the intranasal group,levels of IgG、IgG2a and IgG2b increased obviously on 2～18w,and reached the highest level on 10、4 and 6w respectively;while levels of IgG1、IgG3 and IgE decreased remarkably on 2～18w,and came to the lowest level on 8、12 and 10～12w respectively.In the oral group,levels of IgG、IgG2a and IgG2b rose remakably on 2～18w,and came to the peak value on 14、8 and 8w respectively;while levels of IgG1、IgG3 and IgE decreased on 2～18w,and reached the lowest level on 8、6 and 16～18w respectively.Conclusion：TH1 response could be induced in mice immunized by recombinant BCG-Eg95 vaccine of Echinococcus granulosus in early immunization.