cFLIP、FADD在宫颈癌组织中的表达及意义

Expressions of cFLIP and FADD in cervical carcinoma and their significances

目的:研究cFLIP、FADD在宫颈癌组织中的表达,探讨cFLIP、FADD在宫颈癌发生、发展中的作用及其临床意义。方法:采用免疫组化(SP法)检测64例宫颈癌、15例CIN、12例慢性宫颈炎病例以及10例正常宫颈组织中cFLIP、FADD蛋白的表达,并检测宫颈癌中增殖细胞核抗原标记指数(PCNA-LI)。结果:cFLIP、FADD在宫颈癌中的表达与CIN、慢性炎症组及正常组中的表达差异有显著意义(P<0.01),宫颈癌组织中同时出现cFLIP的高表达和FADD的低表达。cFLIP的表达水平与PC-NA-LI呈正相关(r1=0.7738,P<0.01),FADD的表达水平与PCNA-LI成负相关(r2=-0.8011,P<0.01)。进一步分析发现,在宫颈癌中,肿瘤细胞分化越差,临床分期越晚,以及伴有癌细胞转移的淋巴结中,cFLIP高表达,而FADD低表达。cFLIP、FADD的表达与患者年龄、组织学分型无关。结论:cFLIP、FADD与宫颈癌的发生、发展和转移密切相关,可能是治疗宫颈癌的潜在靶点。

Objective：To investigate the expressions of cFLIP and FADD in cervical carcinoma and explore their associations with the carcinogenesis and development of cervical careinoma,and to evaluate their clinical significances.Methods：The expressions of cFLIP and FADD proteins were detected in cervical tissue samples including 64 cervical carcinoma tissues,15CIN, 12 chronic cervicitis tissues and 10 normal cervix tissues by immunohistochemistry （SP）.And PCNA-LI in 64 cervical carcinoma samples was determined.Results：cFLIP and FADD expressions were significantly different among cervical carcinoma,CIN,chronic cervicitis tissue and normal cervix.The high expression of cFLIP and low expression of FADD were simultaneously found in cervical analysis showed that there was a positive correlation between the level of cFLIP expression and PCNA-LI（r1=0.7738,P〈0.01）,and a negative correlation between the level of FADD expression and PCNA-LI（r2=-0.8011,P〈0.01）in cervical carcinoma tissue.The further analysis found that in cervical carcinoma with the poorer differentiation of tumor cells and at more advanced clinical stage,and in the tumor cells with positive lymph node metastasis,cFLIP expressed high and FADD expressed low.There was no significant correlation of cFLIP and FADD expressions to the age of patients and their histological typos.Conclusions：cFLIP and FADD were closely related to the carcinogenesis,development and metastasis of cervical carcinoma,which indicated that cFLIP and FADD may be the potential targets for treating cervical carcinoma.