扬尘可吸入颗粒物对大鼠支气管肺组织致炎作用的研究

Effect of inhalable particulate matter from roadside dust on airway inflammation in rats

目的:探讨扬尘可吸入颗粒物对支气管肺组织的致炎作用。方法:采集并分析路边扬尘可吸入颗粒物后,染毒大鼠,观察肺组织病理形态的变化,测定肺泡灌洗液中白细胞总数和分类计数及肺匀浆中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和细胞因子诱导的中性粒细胞趋化因子(CINC)。结果:路边扬尘组的病理改变以炎症为主,部分显示出过敏特征。各观察指标与对照组比较有明显差异。结论:路边扬尘可吸入颗粒物可能通过刺激诱导炎性细胞的募集和炎症因子的大量释放,导致炎症反应的发生。

Objective：To study the effect of roadside dust inhalable particulate matter on airway inflammation in rats.Methods： Particles at roadside were collected,analyzed and PM10-treat model of rats were established.Lung tissue histopathology was observed.The counts of total leukocytes and neutrophils in bronchoalveolar lavage fluid （BALF）and superoxide dismutase（SOD）, glutathion peroxidase （GSH-Px）, malondiadehyde （MDA）, cytokin-induced neutrophil chemotactics （CINC）in lung homogenate were measured.Results：The counts of total leukocytes,macrophages and neutrophils in PM10-treat group were increased significantly than those in control group （P〈0.05）.The pathological changes of roadside dust group indicated that it could cause airway injury.Conclusion：The mechanism of the airway inflammation by IP is complicated.The terminal way is inflammatory reaction by inflammatory cells and cell factors.