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用高通量实时荧光PCR技术研究低浓度MNNG诱发的细胞基因应答反应 被引量：4

Study on cellular gene response to low concentration of MNNG in human amnion FL cells by high throughput real-time fluorescent PCR
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摘要 目的:研究低浓度N-甲基-N-硝基-N-甲基亚硝基胍对人羊膜FL细胞部分基因表达的影响,以助于阐明MNNG引起细胞应答反应的基因及其调控机制。方法:用ABI公司的高通量实时荧光定量PCR方法,检测FL细胞在0.2μmol/LMNNG处理后基因表达发生的改变。数据用ABI公司的SDS2.1软件分析。结果:MNNG处理后,在检测的95个基因中,29个基因表达发生改变,其中14个基因下调2倍以上,15个基因下调在1.5-2倍之间;其中有4个基因与细胞周期相关,6个基因与信号转导相关,6个基因与转录调节相关。结论:在低浓度MNNG攻击后,FL细胞的基因表达发生了显著的变化。 AIM： To study the gene expression changes of 95 genes in human FL cells after treatment with low concentration of N - methyl - N＇ - nitro - N - nitrosoguanidine （MNNG） in order to elucidate the response genes and regulatory mechanism of human FL cells exposed to MNNG. METHODS： C, ene expression of human FL cells treated with 0. 2μmol/L MNNG was detected by high throughput real- time PCR. Data were analyzed by SDS 2. 1 software. RESULTS：26 of 95 genes showed significant change in cells after MNNG treatment. The mRNA expression level demore than 2 fold in 14 of 26, and 1.5 to 2 fold in another 15 genes. There were 6 cell cycle related genes, 6 signal transduction related genes and 6 transcription regulation related genes in the 29 genes. CONCLUSION： There are significant alterations of gene profile in human FL cells after low concentration of MNNG attack.
作者 李红娟 石为 卢翔云 邵敏华 周韧 卢大儒 余应年
机构地区 浙江大学医学院病理学和病理生理教研室 复旦大学生命科学院遗传所
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2007年第1期1-6,共6页 Chinese Journal of Pathophysiology
基金 国家重点基础研究发展规划(973)资助项目(No.2002CB512901 No.2002CB512902)
关键词 N-甲基-N’-硝基-N-亚硝基胍 实时荧光定量PCR 基因表达 N- methyl- N＇- nitro- N- nitrosoguanidine Real- time fluorescent PCR Gene expression
分类号 R394.6 [医药卫生—医学遗传学]
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参考文献15

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二级参考文献3

共引文献10

同被引文献39

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中国病理生理杂志
2007年 第1期

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