卡托普利对高血压心脏钙调蛋白磷酸酶与核因子κBp65蛋白表达的影响

Effects of captopril on expression of calcineurin and NF-κB p65 in the heart of hypertensive rats

目的:探讨卡托普利对高血压大鼠心脏钙调蛋白磷酸酶(calcineurin)、核因子κBp65(NFκBp65)蛋白表达影响及其机制。方法:建立腹主动脉缩窄高血压大鼠模型,术后1周用卡托普利治疗,连续10周。以尾动脉测压法观察血压变化,用免疫组织化学和形态计量学方法,观察calcineurin、NF-κBp65在心脏表达和心脏系数的变化。结果:经卡托普利治疗,高血压大鼠血压降低(mmHg,P<0.01),心脏系数显著减小(g/100g,P<0.01),心脏calcineurin和NF-κBp65蛋白高表达下调,阳性表达面积和面积百分比缩小(μm2,P<0.01,或P<0.01)。结论:卡托普利能通过调控信号转导通路calcineurin-NFAT-NF-κBp65关键蛋白表达,逆转高血压心血管重构。

AIM： To study the effect of captopril on ealcineurin and NF - κB p65 in the signal transduction pathway of the cardiovascular remodeling in hypertensive rats. METHODS： Using a animal model of hypertension induced by abdominal aortic banding, the mrs were treated with captopril for 10 weeks. The blood pressure was observed with a tail cuff method. The heart weight and heart weight/body weight were measured. The expression of caleineurin and NF - κB p65 were studied by using immunohistochemistry. RESULTS： After treated with captopril, the blood pressure of the model rats was decreased （P 〈0. 01 ）, the heart weight or heart weight/body weight were also decreased （P 〈0. 01 ）. The calci- neurin and NF - κB p65 protein overexpression was down - regulated, NF - κB - positive area and area percentage were reduced in the heart of hypertensive rats （P 〈0. 01 ,P 〈0. 01 ）. CONCLUSION： Captopril reverses the cardiovascular remodeling by affecting the overexpression of calcineurin and NF - κB p65 involved in the cardiovascular remodeling in hypertensive rats.