阿托伐他汀对自发性高血压大鼠HMG-CoA还原酶表达的影响

Effect of atorvastatin on HMG-CoA reductase expression in spontaneously hypertensive rats

目的:评价阿托伐他汀对自发性高血压大鼠(SHR)HMG-CoA还原酶表达的影响。方法:12只8周龄的SHR随机分为蒸馏水饲养组(SHRDW组,n=6)与阿托伐他汀治疗组(SHRATV组,n=6),并以6只同周龄的正常血压大鼠(WKY)作为对照(WKY组,n=6)。采用RT-PCR与Westernblotting法分别检测HMG-CoA还原酶的mRNA及蛋白表达。同时检测血压与血脂。结果:给药10周后,SHRATV组收缩压显著低于治疗前及SHRDW组(P<0.05),其血清TC、TG、LDL-C及HDL-C的水平与SHRDW组及WKY组相比,也明显降低(P<0.05);SHRATV组HMG-CoA还原酶mRNA的表达水平在给药10周后显著低于WKY组及SHRDW组(P<0.05),其蛋白表达水平同样出现类似的结果。结论:阿托伐他汀能够下调HMG-CoA还原酶的mRNA及蛋白表达水平,不仅使SHR的血脂降低,在某种程度上,还可能与其血压的下降有关。

AIM： To evaluate the effect of atorvastatin on HMG- CoA reductase （HMGR） expression level in spontaneously hypertensive rats （SHR）. METHODS： Twelve eight- week- old SHR was randomized into distilled water group （ SHRDW group, n = 6） and atorvastatin - treated group （ SHRATV group, n = 6）. The age - matched Wistar - Kyoto rats （WKY） were used as control （WKY group, n=6）. RT-PCR and Western blotting were used to detect HMGR mRNA and protein expression levels; respectively. Meanwhile, systolic blood pressure （SBP） and serum lipid levels were examined. RESULTS： Ten weeks a treatment, SBP in SHRATV group was markedly decreased compared with before treatment and SHRDW group （P 〈0. 05）. The levels of TC, TG, LDL- C and HDL- C in SHRAw group were also markedly lower than those in SHRDW group and WKYgroup （P 〈0. 05）. 10 weeks later, HMGR mRNA levels decreased markedly in SHRAw group compared to WKY group andSHRDW group （P 〈0. 05）. The similar results were found in HMGR protein expression levels. CONCLUSIONS： Atorvastatin downregulates mRNA and protein expression levels of HMGR in SHR, which not only decreases the serum lipids levels, but also impacts blood pressure to a certain extent.